The results presented in this study establish an association between phospholipase C-beta (PLC-beta) and tight junction permeability across Madin-Darby canine kidney (MDCK) cell monolayers, an in vitro model for epithelial tissue. These results further show that PLC-beta modulates tight junction permeability by affecting actin filament organization. Hexadecylphosphocholine (HPC) inhibited PLC-b...

Two archaeal Holliday junction resolving enzymes, Holliday junction cleavage (Hjc) and Holliday junction endonuclease (Hje), have been characterized. Both are members of a nuclease superfamily that includes the type II restriction enzymes, although their DNA cleaving activity is highly specific for four-way junction structure and not nucleic acid sequence. Despite 28% sequence identity, Hje and...

The vertebrate tight junction is a critical claudin-based cell-cell junction that functions to prevent free paracellular diffusion between epithelial cells. In Drosophila, this barrier is provided by the septate junction, which, despite being ultrastructurally distinct from the vertebrate tight junction, also contains the claudin-family proteins Megatrachea and Sinuous. Here we identify a third...

OBJECTIVES We sought to create a model of aortic insufficiency in a left heart simulator combined with 3-dimensional echocardiography and finite element modeling of the aortic valve. We examined the effects of aortic root geometry alteration on aortic insufficiency. METHODS Porcine aortic roots were analyzed on a left heart simulator before (control, n = 8) and after intervention (n = 8). Int...

Gap junctional intercellular communication is established when connexin proteins oligomerize into connexon hemichannels, which then pair at the cell surface with connexons from neighboring cells to form functional gap junction channels. Gap junction channels routinely cluster into gap junction plaques, which can exhibit dynamic characteristics while under the frequent processes of formation and...

Ethanol is metabolized into acetaldehyde in most tissues. In this study, we investigated the synergistic effect of ethanol and acetaldehyde on the tight junction integrity in Caco-2 cell monolayers. Expression of alcohol dehydrogenase sensitized Caco-2 cells to ethanol-induced tight junction disruption and barrier dysfunction, whereas aldehyde dehydrogenase attenuated acetaldehyde-induced tight...

Abstract We present a semi-analytical method for computing the reflection and transmission coefficients at joints connecting an arbitrary number of semi-infinite orthotropic plates based on line-junction approximation. use wave approach describe reflection, mode conversion between eigenmodes vibro-acoustic equations plates. A detailed derivation is presented here first time meeting junction wit...

We report electrical and optoelectrical properties of a cross-junction of two semiconducting nanowires. Semiconducting nanowires and their junction play an important role in nanonetwork device. By mechanically manipulating the nanowires, cross-junction nanodevices are fabricated on SiO2/Si substrate using VO2 and ZnO nanowires. These junctions are formed across prepatterned two-probe Au electro...

A systematic theoretical-approach has been made to estimate various parameters, characteristics and their optimization methods for a Josephson junction to be useful as a high-speed switching element in Josephson digital logic electronics. The dynamic response of Josephson junction has been obtained using computer simulation. Our concept of turn-on deay of Josephson junction is applied in order ...

Introduction: Treatment with megavoltage electron beams is ideal for irradiating superficial tumors because of their limited range in tissues. However, for electron treatment of extended areas, such as the chest wall, two or more adjacent fields can be used. Abutment of these fields may lead to significant dose in homogeneities in the junction region. The aim of this study is to offer a new met...