نتایج جستجو برای: keywordschicken sybr green rt

تعداد نتایج: 199695  

Journal: :Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering 2013
Jana Žiarovská Mária Labajová Katarína Ražná Milan Bežo Veronika Štefúnová Tatiana Shevtsova Kateryna Garkava Ján Brindza

The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes-cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time P...

2007
Haukur Gudnason Martin Dufva D.D. Bang Anders Wolff

The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential binding to GC-rich sequences and effects on melting curve analysis. Al...

Journal: :Marine environmental research 2002
T J Dixon J B Taggart S G George

In both laboratory experiments and field investigations with fish a large interanimal variability in CYP1A expression has been observed which may be attributed to variations in environmental inducer exposure and/or inducer response. We are carrying out laboratory investigations to assess the contribution of a potential genetic component in inducer response of flounder (Platichthy sflesus) CYP1A...

Journal: :Iranian journal of biotechnology 2015
Xueqin Rao Jie Sun

BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

2015
Yasuo Suda Mami Nagatomo Risa Yokoyama Mami Ohzono Kazue Aoyama Xu Zhang Kazuhiko Nakajima Naoki Murakami Tadashi Shinoda Tatsuhiko Hirota Sae Yanagihara Jun-Ichiro Nishi

A highly sensitive and convenient method for detecting influenza virus was developed using modified end-point melt curve analysis of a RT-qPCR SYBR Green method and influenza virus-binding sugar chain-immobilized gold-nanoparticles (SGNP). Because SGNPs capture influenza viruses, the virus-SGNP complex was separated easily by centrifugation. Viral RNA was detected at very low concentrations, su...

2015
Elina Khatoon Nagendra Nath Barman Manab Deka Gitika Rajbongshi Kongkon Baruah Nipu Deka Durlav Prasad Bora

Classical swine fever (CSF) is a contagious and devastating viral disease, causing serious losses in the pig industry worldwide. In the control programmes of CSF, rapid detection and identification of the causative agent is a crucial step. Various PCR based techniques like nested RT-PCR, SYBRGreen based RealTime PCR and TaqMan based Real-time PCR were used for detection of CSFV nucleic acid in ...

Journal: :Journal of virological methods 2011
Marcio R T Nunes Gustavo Palacios Keley N B Nunes Samir M M Casseb Lívia C Martins Juarez A S Quaresma Nazir Savji W Ian Lipkin Pedro F C Vasconcelos

Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and ...

2008
Beata Hasiów Natasza Borodynko Henryk Pospieszny

Over a period of few years, Pepino mosaic virus (PepMV) has become one of the most important viral pathogen in tomato production worldwide. So far, five PepMV genotypes (EU, LP, CH2, US2 and US1) have been detected. A real time reverse transcription polymerase chain reaction (RTPCR) procedure, using the fluorescence dye SYBR Green was developed for a rapid and reliable detection of genetically ...

2015
Huazhang Qiu Namei Wu Yanjie Zheng Min Chen Shaohuang Weng Yuanzhong Chen Xinhua Lin

A robust and versatile signal-on fluorescence sensing strategy was developed to provide label-free detection of various target analytes. The strategy used SYBR Green I dye and graphene oxide as signal reporter and signal-to-background ratio enhancer, respectively. Multidrug resistance protein 1 (MDR1) gene and mercury ion (Hg(2+)) were selected as target analytes to investigate the generality o...

2014
Yongbin Zeng Dezhong Li Wei Wang Mingkuan Su Jinpiao Lin Huijuan Chen Ling Jiang Jing Chen Bin Yang Qishui Ou

BACKGROUND Long-term use of nucleos(t)ide analogues can increase risk of HBV drug-resistance mutations. The rtM204I (ATT coding for isoleucine) is one of the most important resistance mutation sites. Establishing a simple, rapid, reliable and highly sensitive assay to detect the resistant mutants as early as possible is of great clinical significance. METHODS Recombinant plasmids for HBV YMDD...

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