In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested...

The blaNDM-1 gene encodes a carbapenemase that confers resistance to almost all β-lactams, including last-resort carbapenems. This is increasingly reported worldwide in nosocomial and community-acquired Gram-negative bacteria. Acinetobacter baumannii is an important opportunistic pathogen that is considered an intermediate reservoir for the blaNDM-1 gene. In this species, the blaNDM-1 gene is l...

Detecting colonization of patients with carbapenemase-producing bacteria can be difficult. This study compared the sensitivity and specificity of a PCR-based method (Xpert MDRO) for detecting blaKPC, blaNDM, and blaVIM carbapenem resistance genes using GeneXpert cartridges to the results of culture with and without a broth enrichment step on 328 rectal, perirectal, and stool samples. The cultur...

Objective(s): Prompt detection of extended-spectrum β-lactamases (ESBL) and carbapenemase-producing enterobacteriaceae is crucial for infection prevention and control strategies. The present study aimed to characterize the ESBL and carbapenemase genes among Enterobacter isolates from an Iranian inpatient population.Materials and Methods:...

Results Between October 2009 January 2010, we identified 1 imported case of KPC (origin, Southern Italy) and 3 cases of NDM-1 producing Enterobacteriaceae, transferred from hospitals in India (1), Pakistan (2) and Serbia/ France(3). Patient 1on admission digestive carrier of E. coli blaNDM-1. Patient 2digestive carrier of P. mirabilis blaNDM-1 detected after extended hospitalization and antibio...

Carbapenems like meropenem and imipenem are being increasingly used for the treatment of multidrug resistant pathogens especially those producing extended spectrum beta lactamase and AmpC beta lactamase. Since the first usage of antibiotics, the burden of resistance has progressively increased over the past several years. Carbapenemases are the enzymes which are capable of hydrolyzing carbapene...

Despite worldwide dissemination of New Delhi metallo-β-lactamase1 (blaNDM-1), outbreaks remain uncommon in China. In this study, we describe the characteristics of the outbreak-related blaNDM-1-producing K. pneumonia isolates in a neonatal unit in Shandong province, China. We recovered 21 non-repetitive carbapenem-resistant K. pneumoniae isolates with a positively modified Hodge test (MHT) or E...

NDM-positive Salmonella sp. strain identified in the United States. Antimicrob Agents Chemother. 2011;55:5957–8. http://dx.doi.org/10.1128/AAC.05719-11 5. Centers for Disease Control and Prevention. Carbapenem-resistant Enterobacteriaceae containing New Delhi metallo-βlactamase in two patientsRhode Island, March 2012. MMWR Morb Mortal Wkly Rep. 2012;61:446–8. 6. Centers for Disease Control and...

Sir, Of the different classes of carbapenemases, class B enzymes are clinically the most significant; they possess the widest substrate hydrolysis range, including penicillins, cephalosporins and carbapenems, but not monobactams. In recent years, metallo-b-lactamase (MBL) genes have spread from Pseudomonas aeruginosa to members of the Enterobacteriaceae. An alarming report by the HPA UK in 2009...