Eggs released by broadcast-spawning marine invertebrates are often negatively buoyant. Blastulae and gastrulae of these species are commonly motile, with passive stability that leads to upward swimming in still water. The earliest occurrence of swimming in developing embryos of diverse invertebrates may therefore permit vertical migration in nature. I used turbulent and laminar shear flows to i...

Elevated shear stress within the skeletal muscle microvasculature is implicated in the induction of a longitudinal splitting form of angiogenesis, which is characterized by the lack of basement membrane breakage. We investigated whether the transcriptional regulator, Ets-1, is responsive to changes in hemodynamic forces and if so, whether Ets-1 controls microvascular endothelial cell integrity ...

Lower-branch traveling waves and equilibria computed in pipe flow and other shear flows appear intermediate between turbulent and laminar motions. We take a step towards connecting these lower-branch solutions to transition by deriving a numerical method for finding certain special disturbances of the laminar flow in a short pipe. These special disturbances cause the disturbed velocity field to...

This study directly measured the relative protein levels in bovine aortic endothelial cells (BAEC) that were cultured for two weeks in normal (5 mM, NG) or high (22 mM, HG) glucose and then were subjected to laminar shear stress at 0 or 15 dynes/cm(2). Membrane preparations were labeled with one of the four isobaric tagging reagents (iTRAQ), followed by LC-MS/MS analysis. The results showed tha...

To investigate the molecular mechanisms of laminar shear stress on the inhibition of apoptosis in endothelial cells, human umbilical vein endothelial cells (HUVECs) were starved in medium containing 2% fetal bovine serum (FBS) and treated with 15 dyne/cm2 shear stress. We confirmed that 15 dyne/cm2 shear stress inhibited the expression of Omi/HtrA2 at the mRNA and protein levels in cultured HUV...

A wind turbine blade's structural dynamic response is simulated and analysed with the goal of characterizing the presence and severity of a shear web disbond. Computer models of a 5 MW offshore utility-scale wind turbine were created to develop effective algorithms for detecting such damage. Through data analysis and with the use of blade measurements, a shear web disbond was quantified accordi...

Physiological levels of laminar shear stress completely abrogate apoptosis of human endothelial cells in response to a variety of stimuli and might therefore importantly contribute to endothelial integrity. We show here that the apoptosis-suppressive effects of shear stress are mediated by upregulation of Cu/Zn SOD and NO synthase. Shear stress-mediated inhibition of endothelial cell apoptosis ...

Direct numerical simulation was performed on two patient-specific abdominal aortic aneurysms (AAA) using physiologically realistic flow conditions. The patients have AAA with diameters of approximately 5 and 7 cm, respectively. The blood flow hemodynamics are shown to consist of large-scale periodic structures and the flow transitions from laminar-to-turbulent and back to laminar during a pulse...

Reendothelialization involves endothelial progenitor cell (EPC) homing, proliferation, and differentiation, which may be influenced by fluid shear stress and local flow pattern. This study aims to elucidate the role of laminar flow on embryonic stem (ES) cell differentiation and the underlying mechanism. We demonstrated that laminar flow enhanced ES cell-derived progenitor cell proliferation an...