A modi®ed two stage in vitro digestion method was used to study the effects of rate of PEG addition (0±1100 mg/g DM) on dry matter (IVDMD) and nitrogen digestibility (IVND) of freeze dried leaf materialfromactivelygrowingshootsofCalliandracalothyrsus (Calliandra)andLeucaenaleucocephala cv. Cunningham (Leucaena). For both species, IVDMD and IVND increased asymptotically with PEG rate; the IVND r...

Field-based bioassays and residue profile analysis were used to determine the relative importance of lethal and sublethal effects of imidacloprid on adult Japanese beetle, Popillia japonica Newman, in blueberries, Vaccinium corymbosum L. Field-based bioassays assessed adult mortality and knockdown, and fruit and leaf injury from Japanese beetles exposed to 4-h and 7-d field-aged residues of imi...

Plants have powerful regenerative abilities that allow them to recover from damage and survive in nature. De novo organogenesis is one type of plant regeneration in which adventitious roots and shoots are produced from wounded and detached organs. By studying de novo root organogenesis using leaf explants of Arabidopsis (Arabidopsis thaliana), we previously suggested that wounding is the first ...

Boerhaavia erecta L. (Nyctaginaceae) is a medicinal weed, indigenous to tropical America, but presently pantropical and appear to constitute a functional leafy vegetable of certain communities. The taxon is reported to possess antiparasitic, antifungal, antibacterial, antiviral, antihistaminic, antioxidant and hepatoprotective properties. The residue of the ethanolic leaf-extract was fractionat...

Value-added traits of alfalfa are needed to provide farmers new high value profitable products. Processing alfalfa to obtain value added products includes three different fractionation methods: 1) wet fractionation; separation into juice fraction and a fiber fraction, 2) dry fractionation; separation into leaves and stems, and 3) fractionation by passage of the whole herbage through the digesti...

Since a safe and effective mass vaccination program against dengue fever is not presently available, a good way to prevent and control dengue outbreaks depends mainly on controlling the mosquito vectors. Aedes aegypti mosquito populations can be monitored and reduced by using ovitraps baited with organic infusions. A series of laboratory experiments were conducted which demonstrated that the ba...

Phylogenetic analysis by maximum likelihood (PAML) has become the standard approach to study positive selection at the molecular level, but other methods may provide complementary ways to identify amino acid replacements associated with particular conditions. Here, we compare results of the decision tree (DT) model method with ones of PAML using the key photosynthetic enzyme RuBisCO as a model ...

Assembly of the dolichol-linked oligosaccharide precursor (Glc(3) Man(9) GlcNAc(2) ) is highly conserved among eukaryotes. In contrast to yeast and mammals, little is known about the biosynthesis of dolichol-linked oligosaccharides and the transfer to asparagine residues of nascent polypeptides in plants. To understand the biological function of these processes in plants we characterized the Ar...

Sisal leaf decortications residue (SLDR) is amongst the most abundant agro-industrial residues in Tanzania and is a good feedstock for biogas production. Pre-treatment of the residue prior to its anaerobic digestion (AD) was investigated using a two-stage pre-treatment approach with two fungal strains, CCHT-1 and Trichoderma reesei in succession in anaerobic batch bioreactors. AD of the pre-tre...