mannan-binding lectin (mbl) is a vital protein of innate immune system and has two critical functions: complement activation through the lectin pathway and opsonization. mbl deficiency has been classified as the most common inherited immunodeficiency known in humans (about 30% of the population), and is associated with predisposition to infections and high risk of some autoimmune diseases. the ...

Aim: Oral cancer is a major health problem in India associated with significant morbidity and mortality. Due to the absence of truly specific symptoms, the early diagnosis of oral cancer is quite challenging. Aberrant glycosylation of cell surface glycoconjugates is an important feature of malignant changes. Abnormal glycosylation in cancer leads to formation of altered glycoconjugates such as ...

The binding of two alpha-galactophilic lectins, Marasmius oreades agglutinin (MOA), and Griffonia simplicifolia I isolectin B(4) (GS I-B(4)) to neoglycoproteins and natural glycoproteins were compared in a surface phase assay. Neoglycoproteins carrying various alpha-galactosylated glycans and laminin from basement membrane of mouse sarcoma that contains the xenogenic Galalpha1-3Gal1-4GlcNAc epi...

Fidelity of glycan structures is a key requirement for biotherapeutics, with carbohydrates playing an important role for therapeutic efficacy. Comprehensive glycan profiling techniques such as liquid chromatography (LC) and mass spectrometry (MS), while providing detailed description of glycan structures, require glycan cleavage, labeling, and paradigms to deconvolute the considerable data sets...

Lectin from the bulbs of Cyclamen mirabile which is an endemic species Turkey was successfully isolated by affinity precipitation with alginate in one step. The purified protein produced two bands showing a dimeric structure SDS-PAGE (13.5 and 14.8 kDa). C. lectin showed activity stability broad pH scale kept its haemagglutination temperature range 4-40°C. MgCl2 HgCl2 inhibited lectin. In this ...

BACKGROUND Blood cultures, and molecular diagnostic tests that directly detect pathogen DNA in blood, fail to detect bloodstream infections in most infected patients. Thus, there is a need for a rapid test that can diagnose the presence of infection to triage patients, guide therapy, and decrease the incidence of sepsis. METHODS An Enzyme-Linked Lectin-Sorbent Assay (ELLecSA) that uses magnet...

Suppressor lymphocytes are known to play an important role in the control of immune responses. The property of the lectin concanavalin-A (Con-A) to induce suppressor cell activity (SCA) has been used as the basis of a method to assess SCA in the peripheral blood of patients. In this study we confirm previous data on the standard double culture assay for Con-A induced SCA and we analyse the poss...

Within the current study, a delivery system based on a novel polymer-lectin conjugate (carbopol-lectin) was evaluated for the oral delivery of therapeutic peptides and proteins. It was demonstrated that covalent attachment of lectin to carbopol does neither decrease nor abolish the specific binding properties of lectin. Bioadhesion studies revealed that liposomes coated with carbopol lectin are...

Ricin is a toxic lectin which presents a potential security threat. Its rapid detection is highly desirable. Here we present a colorimetric bioassay based on the aggregation of carbohydrate-stabilised gold nanoparticles which has been used to detect Ricinus communis Agglutinin 120 (RCA(120)) - a ricin surrogate. To achieve a stable and robust sensing system the anchor chain length and the densi...

The secretory pathway is composed of membrane compartments specialized in protein folding, modification, transport, and sorting. Numerous transient protein-protein interactions guide the transport-competent proteins through the secretory pathway. Here we have adapted the yellow fluorescent protein (YFP)-based protein fragment complementation assay (PCA) to detect protein-protein interactions in...