We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples. Based on the assay, ...

The control of Leishmania infections relies primarily on chemotherapy. The arsenal of drugs available for treating Leishmania infections is limited and includes pentavalent antimonials, pentamidine, amphotericin B, miltefosine, fluconazole and few other drugs at various stages of their development process. In this review, we will discuss the latest results regarding resistance mechanisms to dru...

A series of eight substituted bis-2-hydroxy-1,4-naphthoquinone derivatives was synthesized through lawsone condensation with various aromatic and aliphatic aldehydes under mild acidic conditions. The title compounds were evaluated for antileishmanial activity in vitro against Leishmania amazonensis and Leishmania braziliensis promastigotes; six compounds showed good activity without significant...

The aim of the work is to summarize available literature data on cases visceral leishmaniasis in children registered Russian Federation, as well describe our own clinical observation autochthonous a 9-year-old child, diagnosis which polymerase chain reaction (PCR) method was used. Materials and methods: review domestic foreign over past 20 years, including Internet resources, carried out. We ob...

Every year, leishmaniasis infects from one million to two inhabitants of the Earth. The causative agent are parasites genus protozoan Leishmania, which has more than dozen species. main carriers about 90 species blood-sucking insects. danger current situation on is aggravated by fact that habitat mosquitoes expanding due global warming. Armenia a endemic country. Both imported and local cases d...

Leishmaniasis is a disease caused by a malaria-like parasite called Leishmania in human and some species of animals. Detection of leishmaniasis has always been crucial for control and treatment of the disease. Different strategies have been approached for detection of leishmania. In this review methods used for detection of leishmania infection have been discussed and compared.

General Considerations The most common etiologic agents of VL are 3 species of the L. donovani complex: Leishmania donovani donovani and Leishmania donovani infantum in the Indian subcontinent, Africa, China, the Mediterranean littoral, and the Middle East; and Leishmania donovani chagasi in South America. (Hereafter, organisms will be identified only by genus and species; eg, L. donovani for L...