نتایج جستجو برای: lexsy expression system
تعداد نتایج: 2986708 فیلتر نتایج به سال:
abstract objective: the previous studies indicated that the tlrs especially toll-like receptor 4) tlr4 (are involved in the process of diabetes. the aim of the present research was to investigate the time course expression pattern of tlr4-a main component of innate immune system- in mrna levels in the heart tissue of diabetic male wistar rats. materials and methods: in this research, adult wist...
expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...
Background: CD4+ and CD8+ T cells are the main types of lymphocytes in cell-mediated immunity and play a central role in the induction of efficient immune responses against tumors. The frequencies of T cell subtypes in the peripheral blood and tumor tissues, and draining lymph nodes (dLN) can be considered as useful markers for evaluation of the immune system in cancers. Methods: In this study,...
Introduction: Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in human. PorA is a major component of the outer membrane of N. meningitidis and functions as a cationic Porin. This study aimed to clone and determine the expression of PorA as the first step for producing a proper antigen in a vaccine study against N. meningitidis. Methods: An approximately ...
Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
Objective(s):With regard to pharmacological effects of carvacrol on the respiratory system, its effect on cytokines genes expression in splenocytes of asthmatic mice was examined in this study. Materials and Methods:Splenocytes were isolated from non-sensitized (control group), sensitized mice to ovalbumin (OVA) (group S), and S animals treated with dexamethasone, and three concentrations of c...
b a ckground: quorum-sensing systems regulate expression of several virulence factors in pseudomonas aeruginosa . this study investigated the relation between expression of the las quorum-sensing system and expression of mexy and ampc in35 clinical isolates of p. aeruginosa . m e t hods: antibiotic susceptibility was determined by the disc diffusion method according to the clinical and laborato...
a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
proline dehydrogenase (prodh; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. the goal of this research was gene cloning and characterization of prodh enzyme from pseudomonas fluorescens pf-5 strain. the gene encoding prodh was isolated by means of pcr amplification and cloned in an iptg inducible t...
Background: Pregnancy is a phenomenon that antigens of semi allogenic fetus are in direct contact with mother's immune system. Immune dysregulation can cause fetus rejection by mother's immune system responses. Human leukocyte antigen-G1, as an immunotolerant molecule has a major role to induce tolerance during pregnancy by suppression of natural killer cells through inhibitor receptors on thes...
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