Industrially important extracellular lipase enzyme production was explored by utilizing microbial strain isolated from dairy effluents. Alcaligenes viscosus DOGE-1 strain isolated from dairy waste waters proved to produce extracellular lipase. Various growth factors were attempted to maximize the lipase production by this strain. Growth factors like NH4PO4, Peptone, Urea coupled with peptone, K...

It is known that the enzyme activities of non-target organisms are influenced in vivo or vitro by pesticides play a major role to obtain higher yields field agriculture. foreseen used for various purposes may negatively affect lipase, an digestive system, non target organisms. Therefore, this study, effect diazinon (organophosphate class) and carbaryl (carbamate pesticides, which be widely agri...

Mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. Until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. A predicted lipase (named MAJ1) from marine Janibacter sp. strain HTCC2649 was purified and biochemi...

BACKGROUND The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase from this species. Qualitative analysis of the cell-bound lipase would help to illuminate the regulation mechanism of gene expression and further improve the yield of the cell-...

The aims of this work were to establish improved conditions for lipase production by Candida viswanathii using agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to th...

Aeromonas sp. secrete a lipase/acyltransferase that shares several properties with the mammalian plasma enzyme lecithin:cholesterol acyltransferase. Reaction of the enzyme with tetranitromethane led to modification of 2 tyrosines and a nearly 80% decline in enzyme activity. Replacing Tyr230 with Phe altered the activity of the enzyme in the same way as did treatment with tetranitromethane. Unli...

In recent publications (l-3), the clearing factor of postheparin plasma has been identified with a lipoprotein lipase obtained from rat, rabbit, and calf heart and rat and rabbit adipose tissue. This enzyme catalyzes the hydrolysis of the triglyceride moiety of chylomicrons and low density @-lipoproteins. The hydrolysis of simple triglyceride emulsions is also catalyzed by lipoprotein lipase bu...

Structured lipids (SL) were synthesized by the acidolysis of borage oil with caprylic acid using lipases. Six commercial lipases from different sources and a novel lipase from Pichia lynferdii NRRL Y-7723 were screened for their acidolysis activities and Lipozyme RM IM and NRRL Y-7723 lipase were selected to synthesize symmetrical SL since recently NRRL Y-7723 lipase was identified as a novel c...

Post-heparin plasma in the rat contains triglyceride lipase activity of both hepatic and extrahepatic origin. The lipase released into rat hepatic perfusate by heparin has been characterized in an assay containing Triton X-100, albumin, and [14C]triolein. Fatty acid release was linear for 120 min over a wide range of enzyme concentrations at 27” and 37”. The pH optimum was 9.5. Enzymatic activi...

BACKGROUND Lipases constitute a family of enzymes that hydrolyze triglycerides. They occur in many organisms and display a wide variety of substrate specificities. In recent years, much progress has been made towards explaining the mechanism of these enzymes and their ability to hydrolyze their substrates at an oil-water interface. RESULTS We have determined the DNA and amino acid sequences f...