Present study was aimed to develop and validate a liquid chromatography spectrometry method and pharmacokinetic analysis of acetamiprid in Sprague Dawley rat. For the development of pharmacokinetic method, ChemT was used as internal standard. Extraction and recovery of plasma analyte were done using 0.1% formic acid and acetonitrile solution. An eleven point standard curve was prepared by spiki...

Pharmaceuticals and personal care products (PPCPs) are generally neither totally removed by sewage treatment nor completely destroyed in the environment. Metformin (MET) triclosan (TRI) two compounds PPCPs that have potential to be environmental pollutants. This research aimed determine MET TRI sludge using a liquid chromatograph-mass spectrometer (LCMS-8040) extraction method. The Milli-Q wate...

Growth hormone treatment has gained attention over the past decade as a treatment for heart failure. Human growth hormone (HGH) must be administered by injections (usually daily), so there is considerable advantage to stimulation of endogenous secretion by amino acid-based nutritional supplementation. However, studies investigating the effect of amino acid (AA) supplementation show conflicting ...

Using Liquid Chromatography-Mass Spectrometry (LCMS), profiles of the two isotypes labelled under the same common name Maca deposited in the Medicinal Plant Herbarium, in Australia and Poland, but identified under two different scientific names Lepidium meyenii Walpers (L. meyenii) and Lepidium peruvianum Chacon (L. peruvianum) are presented. The two isotypes correspond to two holotypes of Peru...

During the last decade, significant progress in the analysis of whole genomes and transcriptomes has triggered efforts to analyze the proteome. Advancements in protein extraction, purification, and identification have been driven by the development of mass spectrometers with greater sensitivity and resolution. Nevertheless, comparative and quantitative proteomic technologies have not progressed...

BACKGROUND The present study explores the fungal contamination of important herbal drug raw materials (HDRM), which are widely used in the preparation of many herbal drugs. Understanding of the microbial contamination status of HDRM is one of the important steps to ensure the safety and efficacy of herbal drugs. MATERIALS AND METHODS Eighteen samples of six herbal drug raw materials (HDRM) vi...

Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) biosynthesis and structural composition in a recombinant CHO-S cell line expressing enzymes in the heparin (HP)/(HS) biosynthetic pathway (Dual-10 stably expressing NDST2 and HS3s...

Gandaria (Bouea macrophylla) plants contain antioxidant compounds that can reduce free radicals. Free radicals are one of the causes various degenerative diseases. The purpose this study was to determine fraction had highest activity, and secondary metabolites contained in most active B. macrophylla seeds based on LCMS/MS analysis. Maceration with ethanol solvent. Ethanol maserate concentrated ...

A Shimadzu high performance liquid chromatography – ion trap – time of flight mass spectrometer (LCMS-IT-TOF) was used to aid in the identification of anthocyanin species in rose petals. The effects of varying the parameters which control the collision energy and the collision gas were observed on cyanidin-3,5-diglucoside and its related mono-glucoside and anthocyanidin fragment signals. Furthe...