Undifferentiated, highly chlorophyllous cell cultures; undifferentiated white cell cultures; green, shoot-forming cultures; and white, shoot-forming cultures of Digitalis parpurea L. were established and subcultured every 3 weeks in liquid media in the light or in the dark. The digitoxin content, the chlorophyll content, and the ribulose bisphosphate carboxylase activity of these cultures were ...

The endophytic fungus Berkleasmium sp. Dzf12, isolated from Dioscorea zingiberensis, was found to produce palmarumycins C12 and C13 which possess a great variety of biological activities. Seven biocompatible water-immiscible organic solvents including n-dodecane, n-hexadecane, 1-hexadecene, liquid paraffin, dibutyl phthalate, butyl oleate and oleic acid were evaluated to improve palmarumycins C...

A new fluorescence in situ hybridization assay based on peptide nucleic acid probes (MTB and NTM probes targeting tuberculous and nontuberculous species, respectively) for the identification of Mycobacterium tuberculosis complex and differentiation between tuberculous and nontuberculous mycobacteria (NTM) was evaluated using Lowenstein-Jensen (LJ) solid cultures from 100 consecutive sputum samp...

Although Streptomyces species are major chitin-degraders in soil ecosystems, the expression of the diverse chitinase genes within Streptomyces coelicolor grown in soil has not been assessed. As a first step, the induction pattern of nine chitinase genes in S. coelicolor growing in autoclaved soil was compared with those in liquid cultures. The relative expression levels of nine chitinase genes ...

Using Coreopsis tinctoria liquid cultures of Agrobacterium rhizogenes transformed roots (socalled “hairy roots”) and non-transformed roots have been established. The production of differ­ ent phenylpropanoid compounds by these cultures grown in light or darkness, respectively, was compared. Whereas the composition of phenylpropanoids in the root cultures was not influenced by the culture condit...

A semicomatose 34-year-old man had fever, heart murmur, and multiple gangrenous lesions. Several blood cultures were negative. Cultures of the heart valve grew beta-haemolytic streptococcus group B. The organism was seen in buffy-coat examination of the venous blood. The gas-liquid chromatogram of the serum was different from those of normal controls and of patients with invasive candida infect...

MS liquid medium supplemented with 3 mg/l NAA and 1 mg/l BAP with pH 5.7 was used for suspension cultures. Suspension cultures subjected to biotic elicitors Aspergillus niger, Fusarium oxysporium, Rhizopus oryzae and Penicillium notatum induced increase in forskolin content. Among all the elicitors tried Aspergillus niger proved to be best elicitor at 5% CF which was 6 times higher than the con...

This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by se...

We designed a variable-tilt microfuge fermentation rack to screen liquid cultures of wild and mutant yeasts for ethanol production. The rack design allows for the evaluation of up to 40 cultures in the space normally required for three 125 mL Erlenmeyer flasks. Microfuge tubes containing 1.0 mL of inoculated medium were placed in the rack and incubated with shaking. This technique gave reproduc...

Background and objectives: Silymarin is a unique flavonoid complex with documented hepatoprotective properties. Silybum  marianum hairy root culture as a source for producing silymarin has been an important strategy for study the cell signaling pathway. In the present investigation Trichoderma strains- Silybum marianum hairy root cultures interactions have been studie...