Liquid-liquid phase separation (LLPS) of proteins mediates the assembly biomolecular condensates involved in physiological and pathological processes. Identifying minimalistic building blocks sequence determinant protein is an urgent need but remains challenging partially due to lack methodologies characterize behavior. Here, we demonstrate computational tools efficiently quantify microscopic f...

A continued interest in polyelectrolyte phase diagrams guides the study of interfacial phenomena driven by complexation. The liquid–liquid interfaces formed associative separation oppositely charged synthetic and natural polyelectrolytes provide measurement challenges addressed force-sensitive methods deformed droplet retraction. ultralow tension, typical these systems, is sensitive to salt con...

Liquid-liquid phase separation (LLPS) between tyrosine- and arginine-rich peptides are of biological importance. To understand the interactions proteins in condensed close analogy to complex coacervation, we run multiple umbrella calculations oligomers containing tyrosine (pY) arginine (pR). We find pR-pY complexation be energetically driven. Metadynamics simulations on monomers suggest that th...

A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...

Plastics are one of the most widely used polymeric materials. However, they often undegradable and non-recyclable due to very stable covalent bonds macromolecules, causing environmental pollution health problems. Here, we report that liquid-liquid phase separation (LLPS) could drive formation robust, stable, sustainable plastics using small molecules. The LLPS process sequester concentrate solu...

A novel approach for production of polyetherimide (PEI) microparticles by liquid–liquid phase separation (LLPS) and precipitation is demonstrated the effect used solvents acetophenone dimethyl phthalate on obtained powders outlined. Interestingly, method allows one to produce semi-crystalline particles, while PEI known be hard crystallize typically in amorphous form. To get a deeper understandi...