نتایج جستجو برای: loop bioreactors
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Growing hairy roots in bioreactors has proven challenging. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. Compared to most liquid-phase bioreactors, this gasphase bioreactor offers a completely different environment for growing roots. Design, modeling, and some of the unique engineering aspects inherent to mi...
Tissue engineering involves using the principles of biology, chemistry and engineering to design a 'neotissue' that augments a malfunctioning in vivo tissue. The main requirements for functional engineered tissue include reparative cellular components that proliferate on a biocompatible scaffold grown within a bioreactor that provides specific biochemical and physical signals to regulate cell d...
kLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
Background Orbitally shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). As a consequence, gas transfer issues are thought to limit the effectivene...
CURRENT SCIENCE, VOL. 108, NO. 10, 25 MAY 2015 1782 *A report on the Brainstorming session on ‘Somatic Embryogenesis and Use of Bioreactors’ organized by ICAR-Central Plantation Crops Research Institute, Kasaragod on 2 August 2014. on-line); http://mdgs.un.org/unsd/mdg/ SeriesDetail.aspx?srid=784 12. Hubbell, S. P., In Tropical Forest Diversity and Dynamism (eds Losos, E. C. and Leigh, E. G.), ...
Gradients in industrial bioreactors have attracted substantial research attention since exposure to fluctuating environmental conditions has been shown lead changes the metabolome, transcriptome as well population heterogeneity industrially relevant microorganisms. Such also found impact key process parameters like yield on substrate and productivity. Hence, understanding gradients is important...
Recent advances in molecular and cellular biology have led to the development of technology to engineer plants that are capable of producing a wide variety of products. These include products for pharmaceutical applications, like vaccine antigens, antibodies, antibody-derived fragments and other therapeutic proteins. It is possible to alter endogenous metabolic pathways to obtain new products, ...
JUNE 2008 B ioreactor technologies for mammalian cell culture may seem quite sophisticated (1). In fact, efficient mammalian cell culture is simple and requires just two major elements: mixing and oxygen (O2) transfer. Traditional methods for mixing use an impellor, and the classical O2 transfer method features a sparging stone that expels air bubbles to increase the O2 transfer surface area in...
In aerobic bioprocesses, oxygen is a key substrate; due to its low solubility in broths (aqueous solutions), a continuous supply is needed. The oxygen transfer rate (OTR) must be known, and if possible predicted to achieve an optimum design operation and scale-up of bioreactors. Many studies have been conducted to enhance the efficiency of oxygen transfer. The dissolved oxygen concentration in ...
In recent years, air pollution has become a major worldwide concern. Air pollutants can affect metabolic activity, impede healthy development, and exhibit carcinogenic and toxic properties in humans. Over the past two decades, the use of microbes to remove pollutants from contaminated air streams has become a widely accepted and efficient alternative to the classical physical and chemical treat...
different control strategies of bed temperature and moisture were investigated using various inlet air temperatures and air fluxes in both the ordinary packed bed bioreactor (without cooling water in the jacket) and the bioreactor with cooling water in jacket. the experiments were carried out within a 1-l solid-state packed bed bioreactor in which aspergillus niger was cultivated on wheat bran....
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