Accurate quantification of parasite density in the human host is essential for understanding the biology and pathology of malaria. Semi-quantitative molecular methods are widely applied, but the need for an external standard curve makes it difficult to compare parasite density estimates across studies. Droplet digital PCR (ddPCR) allows direct quantification without the need for a standard curv...

OBJECTIVE The current study aimed to develop a reliable targeted array comparative genomic hybridization (aCGH) to detect microdeletions and microduplications in congenital conotruncal defects (CTDs), especially on 22q11.2 region, and for some other chromosomal aberrations, such as 5p15-5p, 7q11.23 and 4p16.3. METHODS Twenty-seven patients with CTDs, including 12 pulmonary atresia (PA), 10 do...

BACKGROUND Staphylococcus aureus is a common and significant pathogen in cystic fibrosis. We sought to determine if quantitative PCR (qPCR) and 16S rRNA gene sequencing could provide a rapid, culture-independent approach to the identification of S. aureus airway infections. METHODS We examined the sensitivity and specificity of two qPCR assays, targeting the femA and 16S rRNA gene, using cult...

the present study describes the changes in expression of hydroxy- carboxylic acid receptor 2 (hca2 receptor) in femoral epiphysis and metaphysis of rats with glucocorticoid-induced osteoporosis (gio). 16 growing male sprauge dawley rats were randomly divided into two equal groups consisting of normal control and rats that were rendered osteoporotic by receiving 0.1 mg/kg/day dexamethasone throu...

INTRODUCTION Accurate analyses of microbiota composition of low-density communities (10(3)-10(4) bacteria/sample) can be challenging. Background DNA from chemicals and consumables, extraction biases as well as differences in PCR efficiency can significantly interfere with microbiota assessment. This study was aiming to establish protocols for accurate microbiota analysis at low microbial densit...

Mouse 3T3 cells and their Simian Virus 40-transformed derivatives (3T3SV) were used to assess the relationship of transformation, cell density, and growth control to the cellular distribution of newly synthesized glycosaminoglycan (GAG). Glucosamineand galactosamine-containing G A G were labeled equivalently by [all]glucose regardless of culture type, allowing incorporation into the various G A...

reversible logic circuits have found emerging attentions in nanotechnology,optical computing, quantum computing and low power design. a programmablelogic array (pla) is a universal circuit which is used to implement combinationallogic circuits. the main part of a pla is its and array. in this study we propose twotypes of optimized reversible programmable logic array (rpla) circuits. the firstty...