The myogenic determination 1 (MyoD1) gene is a member of the MyoD gene family. It encodes for skeletal muscle-specific transcription factors containing highly conserved basic helix-loop-helix regions that perform important roles in the initiation, maintenance, and regulation of phenotypic traits. We investigated a new single nucleotide polymorphism (SNP) in the MyoD1 gene to evaluate wheth...

Figure 1: E. coli cells engineered with the luxI/luxR system and the microfluidic device setup in our experiments [4]. C6-HSL is a specific type of AHL. Quorum sensing is an essential communication mechanism in bacteria. By transmitting small molecules to each other, bacteria can sense their own population and respond accordingly. Coupling such mechanisms with synthetic biology can lead to a wi...

Burkholderia glumae is a motile plant pathogenic bacterium that has multiple polar flagella and one LuxR/LuxI-type quorum sensing (QS) system, TofR/TofI. A QS-dependent transcriptional regulator, QsmR, activates flagellar master regulator flhDC genes. FlhDC subsequently activates flagellar gene expression in B. glumae at 37°C. Here, we confirm that the interplay between QS and temperature is cr...

Certain gram-negative pathogens are known to control virulence gene expression through cell-cell communication via small diffusible signal molecules termed autoinducers. This intercellular signal transduction mechanism termed quorum sensing depends on the interaction of an N-acylhomoserine lactone (AHL) auto-inducer molecule with a receptor protein belonging to the LuxR family of positive trans...

Numerous gram-negative bacterial pathogens regulate virulence factor expression by using a cell density mechanism termed quorum sensing (QS). An in silico analysis of the Burkholderia mallei ATCC 23344 genome revealed that it encodes at least two luxI and four luxR homologues. Using mass spectrometry, we showed that wild-type B. mallei produces the signaling molecules N-octanoyl-homoserine lact...

Bacterial pheromone signaling is often governed both by environmentally responsive regulators and by positive feedback. This regulatory combination has the potential to coordinate a group response among distinct subpopulations that perceive key environmental stimuli differently. We have explored the interplay between an environmentally responsive regulator and pheromone-mediated positive feedba...

Quorum sensing, the monitoring of population density by bacteria, is used to coordinately control gene expression and therefore particular behaviors under conditions of high cell density. Such group behaviors provide advantages to organisms under certain conditions, such as during pathogenic colonization when virulence traits are induced by a group of bacteria. In the accompanying paper, Lupp a...

The Bohai Bay basin, mainly formed in the Cenozoic, is an important storehouse of groundwater North China Plain. sedimentary deposits transported by paleocurrents often provided favorable conditions for enrichment modern liquid reservoirs. However, due to limited seismic and well logging data, studies focused on macroscopic directions are scarce. In this study, we obtained a series data layers ...