We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the MALDI Bruker Biotyper system (microflex LT; Bruker Daltonik GmbH, Bremen, Germany), on the identification of 50 isolates of clinically encountered molds, including Penicillium marneffei (n = 28), Paecilomyces species (n = 12), Fusarium solani (n = 6), Rhizopus species...

Flavobacteriosis, in Switzerland caused mainly by Flavobacterium psychrophilum (Flavobaceriaceae), has become an important problem for the Swiss fish farming industry. While records of skin and gill infections have been described since a long time, systemic infections, known as the so-called rainbow trout fry syndrome (RTFS), have been reported for the first time not until 1992. Since then, occ...

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a robust and powerful tool for studying biomacromolecules their interactions. However, quantitative detection of high-mass analytes (kDa to MDa range) remains challenging MALDI-MS. Herein, we successfully used commercially available purified proteins (?-galactosidase BSA) as internal standards MALDI-MS analysis achieved...

A rapid and sensitive protein determination method that uses electromembrane extraction (EME) is coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) developed. flat nylon membrane used to collect proteins from an aqueous solution directly analyzed by MALDI/MS after the addition of MALDI matrix. Insulin as a model investigate optimum parameters. The EME conditio...

Nowadays, the use of sulfur dioxide (SO2) during winemaking process is a controversial societal issue. In order to reduce its use, various alternatives are emerging, in particular bioprotection by adding yeasts, with different impacts on yeast microbiota early stages. this study, quantitative-PCR and metabarcoding high-throughput sequencing (HTS) were combined MALDI-TOF-MS monitor population dy...

Imaging mass spectrometry (IMS) is a toolbox of versatile techniques that enable us to investigate analytes in samples at molecular level. In recent years, IMS, and especially matrix-assisted laser desorption/ionisation (MALDI), has been used to visualise a wide range of metabolites in biological samples. Simultaneous visualisation of the spatial distribution of metabolites in a single sample w...

Argentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of Ag+ ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [b(n) + OH + Ag]+, [b(n) - H + Ag]+ and [a(n) - H + Ag]+ ions that are indicative of the C-terminal sequence. These observations paral...

There have been many recent reviews published on MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight) MS (mass spectrometry) for identification of bacteria particularly with relevance to clinical microbiology. MALDI-TOF MS is now a mature technique for bacterial identification with great promise. The purpose of this review is to put into perspective MALDI-TOF MS and other w...

Our team in Europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). To evaluate the utility of MALDI-TOF MS in tropical Africa in collaboration with local teams, we installed an apparatus in the Hôpital Principal de Dakar (Senegal), performed routine identification of ...

Various biochemical, chemical, and microspectroscopic methods have been developed throughout the years for the screening and identification of mutants with altered cell wall structure. However, these procedures fail to provide the insight into structural aspects of the cell wall polymers. In this paper, we present various methods for rapidly screening Arabidopsis cell wall mutants. The enzymati...