BACKGROUND A completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: MALDI TOF: matrix-assisted laser desorption/ionisation time-of-flight. Comparison between results of the new procedure with those obtained by conventional testing is mandatory. METHODS 204 clinical isolates grown on ag...

in this study, 120 aegilops crassa (mmdd genome) accessions from iran were evaluated for high molecular weight proteins. based on sds-page analysis, 17 bands were selected and mass spectrometry was used for protein identification. among 17 bands selected for maldi-tof-tof-ms analyzing, only 4 bands were identified as high molecular weight glutenin subunit similar to aegilops ventricosa and trit...

Mass spectrometry has brought unprecedented possibilities in the field of proteomics. The advances obtained in the last 10 years have been outstanding and have enabled faster and more reliable data acquisition and comparison. One powerful method developed was the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (MALDI-TOF MS profiling) approach, primarily u...

inorganic polyphosphates (polyp) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. four isolates had been screened from soil sample. by maldi-tof analysis, they were identified as bacillius cereus, acinetobacter towneri, b. megaterium and b. cereus. the production of polyp in four isolates was studied in phosphate uptake medium ...

Herein we describe a strategy that combines peptide chips with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to evaluate kinase activities rapidly and semi-quantitatively. We have already shown that monolayers of alkanethiolates on gold are wellsuited as substrates for MALDI-TOF MS, and could be used to measure enzyme activities and to perform hig...

BACKGROUND Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens a...

BACKGROUND Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens. METHODOLOGY/PRINCIPAL FINDINGS An experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella hen...

background : boophilus annulatus is an obligate blood feeder tick that can cause great losses in animals due to anemia and its ability to injure its host skin directly. the aim of this study was identification of cattle humoral immune response to some tick proteins during experimental infestation. methods : immune sera against tick were collected from experimentally infested cattle with ticks. ...