The uptake of maltose and related sugars in Gram-negative bacteria is mediated by an ABC transporter encompassing a periplasmic component (the maltose-binding protein or MalE), a pore-forming membrane protein (MalF and MalG) and a membrane-associated ATPase (MalK). In the present study, the structure determination of the apo form of the putative maltose/trehalose-binding protein (Xac-MalE) from...

The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resistance, failed to produce acid from maltose. The same proportion of naturally ...

In 1950 Pan and his associates (1) reported the conversion of maltose into non-fermentable sugars by an enzyme solution obtained from Aspergillus niger. They isolated a crystalline, non-fermentable, reducing trisaccharide from the reaction mixture (2). Structural studies by French (3) and by Wolfrom et al. (4) have shown this trisaccharide to be a 4-a-isomaltosyl-n-glucose (panose). In a prelim...

The maltose transporter MalFGK(2) of Escherichia coli is a member of the ATP-binding cassette superfamily. A periplasmic maltose-binding protein (MBP) delivers maltose to MalFGK(2) and stimulates its ATPase activity. Site-directed spin labeling EPR spectroscopy was used to study the opening and closing of the nucleotide-binding interface of MalFGK(2) during the catalytic cycle. In the intact tr...

background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...

to evaluate the flour quality 10 flour plants were selected in iran, tehran province. two kinds of flour were sampled from each plant (flour with 87% extraction named straight-run- flour as well as patent flour). samples were analyzed for their moisture content, ash, maximum viscosity of dough, particle size, falling number and the amount of damaged starch, which was determined through maltose ...

The maltose transporter of Saccharomyces cerevisiae is rapidly degraded during fermentation in the absence of a nitrogen source. The location and mechanism of degradation of the transporter have been investigated. Using mutants defective in endocytosis, we have shown that degradation of this transporter requires internalization by endocytosis. In addition, studies of mutants defective in protea...

Microspore cells develop into male gametophytes, which are released as pollen. Under certain stress conditions, the developmental pathway of microspores can be transformed an embryo instead pollen with haploid technology. In this experiment, 2 pepper breeding lines (G-1 and G-2) 4 nutrient media formed Murashige & Skoog (1962-MS) + 30 g L-1 sucrose or maltose without vitamins antioxidan...

In a cell-free system programmed with a plasmid bearing a malP'-'lacZ gene fusion under the control of malPp, beta-galactosidase synthesis was strictly dependent on the presence of both the MalT activator protein and the inducer of the Escherichia coli maltose regulon. We show that, among all maltodextrins tested (from maltose to maltoheptaose), only maltotriose was able to induce beta-galactos...