نتایج جستجو برای: mammalian cell culture

تعداد نتایج: 1924240  

2009
Katarina Blagović Salil P. Desai Joel Voldman

Adherent mammalian cells dynamically interact with their extracellular matrix (ECM) and culture substrate. To accommodate this sensitivity, standard culture techniques typically utilize tissue culture polystyrene (TCPS), a treated polystyrene substrate that promotes cell attachment. However, TCPS cannot be easily integrated into microfluidic devices as it is incompatible with conventional fabri...

Mehdi Hajian Mohammad Hossein Nasr Esfahani Mohsen Forozanfar Parvaneh Abedi Sayyed Mortaza Hosseini Vajihe Asgari

Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...

2011
Stéphanie TISSOT Mohamed Farhat Martino Reclari Matthieu Dreyer Nicola Parolini Maria de Jesus

Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in particular against cancer and autoimmune disorders. Most of the mAbs are produced as recombinant proteins by mammalian cells which ensure human-l...

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