this study presents the first details on morphological and histological characteristics of gonads, gonadal development stages and reproductive index of the persian loach, oxynoemacheilus persa (heckel, 1847), an iranian endemic species. sampling was done from april 2010 to april 2011 using electrofishing device and a total of 196 individuals were collected. the gonads of specimens were removed,...

background: ovarian tissue transplantation is emerging technologies for fertility preservation. in addition, in vitro maturation (ivm) of oocytes retrieved from ovarian tissues may overcome the fertility defects in certain cases. objective: the aim was to evaluate the best site for ovarian tissue transplantation in mice. also, feasibility of ivm of oocytes retrieved from auto grafted ovarian ti...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...

background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

The effects of different preservation conditions (temperature 20-25 or 10-15 degrees C; duration 12 or 24 h) for ovaries of Cervus nippon yesoensis on in vitro maturation of oocytes were examined. When ovaries were kept for 12 h at 20-25 degrees C, maturation rate of oocytes was highest (71%); however, it declined when the preservation time was extended to 24 h (31%). When the preservation temp...

Dendritic cells (DC) are central regulators of immunity. Signal-induced maturation of DCs is assumed to be the starting point for specific immune responses. To further understand this process, we analyzed the alteration of transcript profiles along the time course of CD40 ligand-induced maturation of human myeloid DCs by Affymetrix GeneChip microarrays covering >6800 genes. Besides rediscovery ...

background: mesenchymal stem cells (mscs) possess a wide range of immunomodulatory functions mostly in immune cells including dendritic cells (dcs). dcs are the key cells in the immune response and play an important role in initiating cell-mediated immunity. objective: to evaluate the immunomodulatory effects of mscs supernatant on maturation and function of dcs. methods: bone marrow derived mi...

we review abscess management by ibn al-quff in the 12 century, described in his book al omdaa (arabic version). he explained in detail how an abscess presents, access for drainage, the proper preparation by the surgeon and patient for a procedure, and technical guidelines. it is considered advanced in ensuring the best results of managing this pathology. much of his advice is already in use, an...

Human pluripotent stem cells derived cardiomyocytes (PSC-CMs) have been widely used for disease modeling, drug safety screening, and preclinical cell therapy to regenerate myocardium. Most studies have utilized PSC-CM grown in vitro for a relatively short period after differentiation. These PSC-CMs demonstrated structural, electrophysiological, and mechanical features of primitive cardiomyocyte...

The effects of maturation stages on some physical and chemical properties the jujube fruits were investigated. described as green, yellow, half-red red maturation. composition fruit significantly changed depending stage. size weight increased, peel color from green to with content water-soluble vitamins, trans-resveratrol, total phenolic antioxidant capacity decreased during trans-resveratrol w...