Rodent organotypic hippocampal cultures spontaneously develop epileptiform activity after approximately 2 weeks in vitro and are increasingly used as a model of chronic post-traumatic epilepsy. However, organotypic cultures are maintained in an artificial environment (culture medium), which contains electrolytes, glucose, amino acids and other components that are not present at the same concent...

Okra (Abelmoschus esculentus) is an important vegetable that has been indigenized in African countries where it consumed as a relish. There still, however, lack of documented cultivation techniques for better yields. An organic medium-enclosed trough (OMET) system growing technique developed to reduce water and nutrient seepage during plant production. The study objectives evaluated the effect ...

introduction: determination of best culture medium for fertilization and development of nmri mice. material and methods: development of fertilized mature oocytes of nmri mice was studied for five days in several culture media (ksom, t6, m16, czb, g1tm ver3 and g2tm ver3) following hormone therapy with pmsg and hcg. results: the rate of fertilization after 24 hours was 97.4% in ksom, 83.5% in t6...