نتایج جستجو برای: melt curve technique
تعداد نتایج: 744772 فیلتر نتایج به سال:
Evaluation of PCR and high-resolution melt curve analysis for differentiation of Salmonella isolates
Compared to the reported method, relatively high pure silver sulfide iodide (Ag3SI) crystal, a kind of super-ionic conductor, was synthesized in a more facile procedure through the designed melt-cooling technique followed by a heat-treatment at 160°C for half an hour, which is confirmed by the corresponding X-ray Diffraction patterns. Utilizing the designed differential thermal analyzer method,...
This study sought to determine whether the quality of enzyme preparations can be determined from their melting curves, which may easily be obtained using a fluorescent probe and a standard reverse transcription-polymerase chain reaction (RT-PCR) machine. Thermal melt data on 31 recombinant enzymes from Plasmodium parasites were acquired by incrementally heating them to 90 degrees C and measurin...
Multiple drug resistance in Salmonella is an emerging problem in the area of food safety. Depending on the virulence and antibiotic resistance characteristics of the Salmonella strain, infections of varying severity could result. In this study, a multiplex melt curve real-time PCR assay for the detection of virulent and antibiotic resistance strains of Salmonella was developed with two primer s...
We numerically simulate the field measurements of Rayleigh surface waves and electrical resistivity in which target depth is set to be less than 50-m. The are simulated terms fundamental mode group phase velocities. seismic data assumed collected through a conventional shot-gather. velocities found from application multiple filter technique single-station fashion while for slant stacking, or li...
The viscoelastic character of polymer solutions and melts gives rise to instabilities that are not seen in the flows of Newtonian liquids. In industrial applications such as coating and extrusion, these so-called “elastic” instabilities can impose a limitation on the throughput. Hence, it is important to understand, and if possible, to suppress them. The first instability we study is the phenom...
Anthracene fibers are grown inside a microstructured quartz matrix to form a multicore optical fiber for X-ray detection. A modified fiber growth method for single crystal anthracene from the melt via the Bridgman-Stockbarger technique is presented. The anthracene fiber is characterized by using spectrophotometry, Raman spectroscopy, and X-ray diffraction. These results show the anthracene grow...
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