Analogous to metagenomics, environmental transcriptomics (metatranscriptomics) retrieves and sequences environmental mRNAs from a microbial assemblage without prior knowledge of what genes the community might be expressing. Thus it provides the most unbiased perspective on community gene expression in situ. Environmental transcriptomics protocols are technically difficult since prokaryotic mRNA...

BACKGROUND AND AIMS The aim of this study was to develop species-specific molecular markers for Bambusa balcooa and B. tulda to allow for their proper identification, in order to avoid unintentional adulteration that affects the quality and quantity of paper pulp production. METHODS Two putative, species-specific RAPD markers, Bb836 for B. balcooa and Bt609 for B. tulda were generated using a...

A very sensitive and specific method for the random amplification of whole DNA molecules and genomes ranging from 400 base pairs (bp) to 40 Megabase (Mb) is described. This simple, two step PCR (1-3) strategy utilizes tagged random primers that consist of a pool of all possible 3' sequences for binding to the target DNA and a constant 5' region for the detection of incorporated primers. As litt...

UNLABELLED PREMISE OF THE STUDY New microsatellite primers were developed for the diploid herb Anthyllis vulneraria. These primers will be used in upcoming studies focusing on random genetic variation, local adaptation, and phenotypic plasticity in alpine plants. • METHODS AND RESULTS The new primers were adjusted to separate PCR amplicons (70 to 170 bp) on precast Spreadex gels using hori...

Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and seque...

The usefulness of random amplified microsatellite polymorphism markers to study the genetic diversity and relationships among cultivars belonging to Prunus salicina and P. domestica and their wild relatives (P. insititia and P. spinosa) was investigated. A total of 226 of 234 bands were polymorphic (96.58%). The 226 random amplified microsatellite polymorphism markers were screened using 15 ran...

A total of 65 blood samples collected from Holstein cattle were employed for DNA extraction. Genomic DNA were amplified by means of random amplified polymorphic DNA (RAPD). One hundred and one random primers (Operon kits OPAA, OPAO, OPAV, OPC, OPE and OPA-06) were used for polymerase chain reactions (PCR). The PCR products were size fractionated by means of electrophoresis in agarose gel, trans...

DNA based RAPD (Randomly Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in number of crop plants. In this study, 44 okra genotypes collected from different parts of India, were selected to assess genetic distinctiveness and relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 14 arbitrary 10 mer primers. The molec...

Assigbetse, K. B., Fernandez, D., Dubois, M. P., and Geiger, J.-P. 1994. Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random amplified polymorphic DNA (RAPD) analysis. Phytopathology 84:622-626. We used pathogenicity and random amplified polymorphic DNA (RAPD) markers to assess genetic diversity among 46 isolates of Fusarium ox~~sporum f. sp. vasinfectuni of world...

Inferred amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from five different methanogen species were aligned and two regions with a high degree of homology flanking a more variable region were identified. Analysis of the DNA sequences from the conserved regions yielded two degenerate sequences from which a forward primer, a 32-mer, and a reverse primer, a 23-mer, could be de...