Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...

The nonhematopoietic stromal cells of the bone marrow are critical for the development of hematopoietic stem cells into functionally competent blood cells. This study addresses the question of whether bone marrow stromal cell cultures in the Dexter system propagate multiple different mesenchymal stromal cell types or one stromal cell type that expresses multiple phenotypes. Results show that is...

Over the last few years, the mesenchymal stromal compartment of the bone marrow has regained interest. The main reason for this resurgence is the recognition of their immunomodulatory properties and their prominent role in the maintenance and regulation of hematopoiesis. This has simultaneously prompted preclinical and clinical studies trying to take advantage of these properties and, at the sa...

Human bone marrow (BM) contains a population of non-hematopoietic stem cells also termed stromal cells, mesenchymal cells or multipotent mesenchymal stromal cells (MSCs). These cells have unique stem cell-like properties including their ability to self-renew, differentiate into multiple tissue types, and modulate immune cell responses through paracrine effects. These properties have positioned ...

The endometrium is central to mammalian fertility. The endometrial stromal cells are very dynamic, growing and differentiating throughout the estrous cycle and pregnancy. In humans, stromal cells appear to have progenitor or stem cell capabilities and the cells can even differentiate into bone. It is not clear whether bovine endometrial stromal cells exhibit a similar phenotypic plasticity. So,...

introduction demyelination is a common lesion of spinal cord injury; cell therapy is an approach for replacing the lost oligodendrocytes. objective in this study, bone marrow stromal cells (bmscs) have been transdifferentiated into oligodendrocyte-like cells (olcs) and used in cytotherapy of contused spinal cords in rats. materials and methods the bmscs were collected from rat long bones, cultu...