Skyscraper running is here analyzed in terms of mechanical and metabolic requirements, both at the general and at the individual level. Skyscraper runners' metabolic profile has been inferred from the total mechanical power estimated in 36 world records (48-421 m tall buildings), ranked by gender and age range. Individual athlete's performance (n=13) has been experimentally investigated during ...

2009;22(1) S Afr J Clin Nutr a Hattingh Z, PhD a Walsh C, PhD b Veldman FJ, PhD c Bester CJ, BScHons a Department of Nutrition and Dietetics, University of the Free State, b Fibrinogen Unit, Central University of Technology, Free State, c Department of Biostatistics, University of the Free State Correspondence to: Zorada Hattingh, e-mail: [email protected]

Multicomponent analyses were carried out for three types of urinary constituents: steroids, acids, and drugs and drug metabolites. The methods were based on gas-phase analytical techniques, which include the use of instruments and instrumental systems for gas chromatography, gas chromatogra phy-mass spectrometry, and mass spectrometry-com puterization. After isolating an analytical sample, we p...

Genetic diversity of phylogenetic or functional markers is widely used as a proxy of microbial diversity. However, it remains unclear to what extent functional diversity (FD), gene sequence diversity and community functioning are linked. For a range of denitrifying bacteria, we analyzed the relationships between (i) the similarity of functional traits evaluated from metabolic profiles (BIOLOG p...

BACKGROUND Although the effects of acute dietary interventions on the human metabolome have been studied, the extent to which the metabolome can be normalized by extended dietary standardization has not yet been examined. OBJECTIVE We examined the metabolic profiles of healthy human subjects after extended dietary standardization to see whether the inherent variation in the human metabolome c...

Cells and organisms are regularly exposed to a variety of stresses, and effective responses are a matter of survival. The article describes a multi-scale experimental and dynamical modeling analysis that clearly indicates concerted stress control in different temporal and organizational domains, and a strong synergy between the dynamics of genes, proteins and metabolites. Specifically, we show ...

We investigated the metabolic profile of cancer stem cells (CSC) isolated from patients with epithelial ovarian cancer. CSC overexpressed genes associated with glucose uptake, oxidative phosphorylation (OXPHOS), and fatty acid β-oxidation, indicating higher ability to direct pyruvate towards the Krebs cycle. Consistent with a metabolic profile dominated by OXPHOS, the CSC showed higher mitochon...

The post-genomics era has provided researchers with access to a new generation of tools for the global characterization and understanding of pathogen diversity. This review provides a critical summary of published Leishmania post-genomic research efforts to date, and discusses the potential impact of the addition of metabolomics to the post-genomic toolbox. Metabolomics aims at understanding bi...

Medical nutrition therapy for people with diabetes should be individualized, with consideration given to the individual's usual food and eating habits, metabolic profile, treatment goals and desired outcomes. Monitoring of metabolic parameters, including glucose, HbA1c, lipids, blood pressure, body weight, and renal function, when appropriate, as well as quality of life is essential to assess t...

We investigate the ability of algorithms developed for reverse engineering of transcriptional regulatory networks to reconstruct metabolic networks from high-throughput metabolite profiling data. For benchmarking purposes, we generate synthetic metabolic profiles based on a well-established model for red blood cell metabolism. A variety of data sets are generated, accounting for different prope...