Thymic epithelial cells (TECs) support T cell development in the thymus. Cortical thymic epithelial cells (cTECs) facilitate positive selection of developing thymocytes whereas medullary thymic epithelial cells (mTECs) facilitate the deletion of self-reactive thymocytes in order to prevent autoimmunity. The mTEC compartment is highly dynamic with continuous maturation and turnover, but the gene...

BACKGROUND The expression of miR-205 is closely related to the occurrence, development, and prognosis of lung cancer and breast cancer. However, studies show that it plays opposite roles in different tumor types. Because the expression and regulation of miR-205 are primarily confined to epigenetic areas, whether genetic variation of miR-205 is related to the occurrence or to the development of ...

OBJECTIVES We investigated the role of miR-205 in the osteogenic differentiation of vascular smooth muscle cells (VSMCs). METHODS Osteogenic differentiation of human aortic smooth muscle cells (HASMCs) was induced by 10 mM β-glycerophosphate (β-GP). Alizarin Red S staining, alkaline phosphatase (ALP) activity and osteocalcin secretion were used to determine osteogenic differentiation of HASMC...

MicroRNAs (miRNAs) are endogenously small non-coding RNAs which are key post-transcriptional regulators of gene expression. Deregulation of miRNAs is common in human tumorigenesis. We report that miRNA-205 is significantly down-regulated in glioma cell lines and tissue specimens. Ectopic expression of miRNA-205 induces apoptosis, cell cycle arrest...

Myeloid/lymphoid or mixed-lineage AF4 acute lymphoblastic leukemia (MLL-AF4 ALL) is a pediatric leukemia that occurs rarely in adults. MLL-AF4 ALL is typically characterized by the presence of chromosomal translocation (t(4;1l)(q21;q23)), leading to expression of MLL-AF4 fusion protein. Although MLL-AF4 fusion protein triggers a molecular pathogenesis and hematological presentations that are un...

BACKGROUND Ovarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs (miRNAs or miRs), some of which have oncogenic or tumor suppressor properties. Several studies suggested that m...

Prostate cancer (PCa) is the most common type of cancer in men in the United States, which disproportionately affects African American descents. While metastasis is the most common cause of death among PCa patients, no specific markers have been assigned to severity and ethnic biasness of the disease. MicroRNAs represent a promising new class of biomarkers owing to their inherent stability and ...

To investigate the effect of pristimerin on gefitinib resistance in lung cancer cells and its regulation microRNA-936. Lung cell HCC827 was cultured vitro, resistant HCC827/gefitinib established were randomly assigned to control group, pristimerin-L pristimerin-M pristimerin-H gefitinib+pristimerin gefitinib+microRNA-negative gefitinib+microRNA-936 gefitinib+pristimerin+anti-microRNA negative g...

To investigate the effect and molecular mechanism of vitexicarpin on biological behavior colorectal cancer cells is objective study. The human epithelial cell line Caco-2 were divided into control group, low, middle high dose groups vitexicarpin, plasmid cloning DNA-circ_0000419 DNA anti-microRNA-224 anti-microRNA-224-negative vitexicarpin+small interfering-circ_0000419 group vitexicarpin+micro...