The stress corrosion cracking (SCC) of stainless steel is one of the biggest problems for maintaining atomic power and chemical plants. However the mechanism has not been solved because of difficulty in observing hydrogen movement. In order to solve this problem, the author has developed a new SCC test method that enables the super Kelvin force microscope (SKFM) and the Kelvin force microscope ...

Fast and low phototoxic imaging techniques are pre-requisite to study the development of organisms in toto. Light sheet based microscopy reduces photo-bleaching and phototoxic effects compared to confocal microscopy, while providing 3D images with subcellular resolution. Here we present the setup of a light sheet based microscope, which is composed of an upright microscope and a small set of op...

Lens-free holographic microscopy can achieve wide-field imaging in a cost-effective and field-portable setup, making it a promising technique for point-of-care and telepathology applications. However, due to relatively narrow-band sources used in holographic microscopy, conventional colorization methods that use images reconstructed at discrete wavelengths, corresponding to e.g., red (R), green...

We propose a method that recovers phase from a stack of through-focus intensity images taken with a partially coherent microscope employing illumination of any arbitrary source shape. Our algorithm uses a Kalman filtering approach, which is fast, accurate and robust to noise. We validate the method in simulation and with experimental data taken on a commercial microscope with varying condenser ...

This preparation would maintain good contrast and reduce the formation of lead carbonate in resin-embedded tissue sections. new method provides a shorter staining protocol fewer than 5 minutes, extended storage duration, improved contrast, higher broad differential range, prevents contamination from environment. Introducing 20% ethanol to citrate formula makes it be more stable nitrate formula.