Thrombospondin (TSP), a multifunctional a-granule glycoprotein of human platelets binds fibrinogen, fibronectin, heparin, histidine-rich glycoprotein (HRGP), and plasminogen (Pig), and thus, may play an important role in regulating thrombotic influences at vessel surfaces. In this study we have demonstrated that purified human platelet TSP formed a trimolecular complex with human Pig and HRGP. ...

Isolates were cultured in an TYM-medium (MEINGASSNER and HEYWORTH 1982) containing antibiotics for inhibition of bacterial and fungal growth. Inhibitors were omitted from the growth medium when the cultures proved to be free from contaminants. Drug testing on the isolates was usually performed within 10 passages after isolation. The laboratory procedure for susceptibility testing to metronidazo...

Virus-neutralizing antibodies are seldom estimated in routine diagnostic laboratories because methods involving replicate series of tubed cell monolayers are so demanding of time and materials. Simplified methods for performing neutralization tests have been described, but these were generally developed for specific purposes and have required various forms of specialized apparatus. One of the e...

BACKGROUND Recombinant Fab fragments are attractive as reagents for novel sandwich immunoassays, but no such assays have been described. We developed a dual-label two-site immunoassay based entirely on recombinant Fab fragments and compared it to the same assay with intact monoclonal antibodies. METHODS The capture Fab fragment, which binds free prostate-specific antigen (PSA) and PSA in comp...

We have developed an enzyme immunoassay to measure atazanavir (ATV) levels in plasma and cells. Anti-ATV polyclonal antibodies were raised in rabbits by using a synthetic ATV derivative coupled to bovine serum albumin as the immunogen, and the enzyme tracer was prepared by chemically coupling the ATV derivative with acetylcholinesterase. These reagents were used to develop a sensitive competiti...

Prostate-specific antigen (PSA) and human prostatic glandular kallikrein (hK2) have 79% identity with the primary structure. When we used recombinant hK2 protein, only 7 of 23 monoclonal anti-PSA IgGs (monoclonal antibodies, MAbs) cross-reacted with hK2, which enabled us to design a novel immunofluorometric MAb-MAb assay for the specific detection of hK2. In the first incubation, an excess of M...

The objective of this study was to develop a rapid and reliable method for flow cytometric analysis of porcine whole blood cells. Fifty-microliters of heparin- or EDTA-treated whole blood was added to wells of a round-bottom 96-well microtitration plate. Each well contained 10 microl of an appropriate dilution of four different antibodies (40 microl total; two primary monoclonal antibodies and ...

BACKGROUND We have demonstrated that vaccination with pDNA encoding cysteine proteinase Type II (CPA) and Type I (CPB) with its unusual C-terminal extension (CTE) can partially protect BALB/c mice against cutaneous leishmanial infection. Unfortunately, this protection is insufficient to completely control infection without booster injection. Furthermore, in developing vaccines for leishmaniasis...

In food microbiology, novel strategies to fight foodborne pathogens are certainly welcome. In this data report, cationic nanostructures built from combinations of nanoparticles, antimicrobial peptide and cationic lipid are evaluated against important foodborne pathogens such as Escherichia coli, Salmonella enterica subsp. serovar Typhymurium, Staphylococcus aureus and Listeria monocytogenes. Th...

Background: Studies with longitudinal and/or time-series designs would benefit from non-invasive methods to assess immune response. Immune response levels typically are measured with blood obtained by venipuncture, which is distressing to subjects and, thus, may adversely affect recruitment and retention of subjects in studies with multiple or repeated biological sampling. Aims: The study aims ...