Hookworms of the genus Ancylostoma secrete an anticoagulant that both inhibits the clotting of human plasma and promotes fibrin clot dissolution. This anticoagulant activity is attributable to a 36,000 dalton proteolytic enzyme. The protease can degrade fibrinogen into five smaller polypeptides that intrinsically have anticoagulating properties, covert plasminogen to a mini-plasminogen-like mol...

The composition and physical properties of raw milk from a commercial herd were studied 8 over a one year period in order to understand how best to utilise milk for processing 9 throughout the year. Protein and fat levels demonstrated seasonal trends, while minerals and 10 many physical properties displayed considerable variations, which were apparently unrelated 11 to season. However, rennet c...

Administration of vitamin K-antagonists induces the appearance of abnormal clotting factors in the blood (man, cow) or in the l iver (rat). These abnormal clotting factors (descarboxyfactors) contain glutanfc acid (G1u) residues instead of the 7-carboxyglutamic acid (Gia) residues, which occur in normal clotting factors [1]. In human and bovine plasma -90% of the abnormal proteins consists of d...

background there are different types of aflatoxins, including aflatoxin m1, m2, g1, g2, b1 and b2 produced in the food chain, especially when the food is infected with fungi and can directly be consumed by humans. considering that our country is located in the subtropical geographic region where temperature and humidity ecological factors are among determining factors in contamination with afm1...

Purpose: To further investigate the binding of α−crystallin to other proteins as part of its chaperone-like activity, we studied interactions of α−crystallin with recombinant calf prochymosins and chymosin. Methods: Recombinant calf prochymosin B and one C-terminal mutant (PC+2, with two additional residues, Histidine-Glycine) were expressed as inclusion bodies in E. coli. Native and mutant pro...

The composition and physical properties of raw milk from a commercial herd were studied over a 1 year period in order to understand how best to utilise milk for processing throughout the year. Protein and fat levels demonstrated seasonal trends, while minerals and many physical properties displayed considerable variations, which were apparently unrelated to season. However, rennet clotting time...

Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for heterologous chymosin production for the following reasons: (1) it is considered...

The topic of this thesis is the simulation of blood clotting using the Lattice Boltzmann method and taking into account the non-Newtonian behavior of blood. This kind of simulations could be used for the treatment of diseases like stenosis and aneurysms. Their field of application includes the diagnostic of the diseases, the planning of surgeries and the investigation of new medical treatment s...

Milk coagulation properties (MCP) are fundamental in cheese production, particularly in countries where a large amount of milk is destined to cheese industry. Several studies have demonstrated the role of MCP on cheese yield and quality. Lasting years, a general worsening of MCP at the herd and animal level has been detected. The coagulation of milk is influenced by several factors such as type...