derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (pdc). the inhibition of transketolase, a thiamine requiring enzyme such as pdf, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (mnpb) is reported here. these studies indicate that the inhibitor binds to the enzyme at the active site. a two-step inhibition was observed, first the in...

This study elucidated the role of boeravinone B, a NorA multidrug efflux pump inhibitor, in biofilm inhibition. The effects of boeravinone B plus ciprofloxacin, a NorA substrate, were evaluated in NorA-overexpressing, wild-type, and knocked-out Staphylococcus aureus (SA-1199B, SA-1199, and SA-K1758, respectively). The mechanism of action was confirmed using the ethidium bromide accumulation and...

Antibiotic resistance has become a global health problem for humankind. Improper use of antibiotics resulted in the increasing evolved bacterial to them. There are different types mechanisms including efflux pumps. To overcome pump activity on drugs, combinatorial therapy existing antimicrobials with natural products is promising insight prevent multidrug resistance. In this study, inhibitory a...

This study sought to give a scientific basis to plants already used for traditional purposes and also probe new antibacterial constituents from randomly selected plants whose anti-infective properties have not been evaluated. The antibacterial activity of ethanolic extracts from 19 Zimbabwean plants was assessed using the agar diffusion assay, minimum inhibitory concentration and minimum bacter...

Boosted protease inhibitor regimens combine ritonavir with a second, 'boosted' protease inhibitor to enhance patient exposure to the latter agent, thereby preventing or overcoming resistance and allowing less frequent dosing, potentially improving adherence. The advantages offered by ritonavir boosting are primarily attributable to the drug's pharmacokinetic properties. Ritonavir's inhibition o...