BACKGROUND The Chlamydia trachomatis incidence rate in Finnmark, the most northern and sparsely populated county in Norway, has been twice the national average. This population based cross-sectional study among Finnmark high school students had the following aims: i) to examine distribution of multilocus sequence types (STs) of C. trachomatis in a previously unmapped area, ii) to compare chlamy...

The pathogenic yeast Candida dubliniensis is phylogenetically very closely related to Candida albicans, and both species share many phenotypic and genetic characteristics. DNA fingerprinting using the species-specific probe Cd25 and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal gene cluster previously showed that C. dubliniensis is comprised of three major c...

Conserved single-nucleotide polymorphisms (SNPs) which characterize the allelic profile of the major epidemiological lineage ST-21 were identified from the alleles within the current Campylobacter jejuni multilocus sequence typing (MLST) database. Allelic discrimination assays were designed for the detection of SNPs, enabling rapid strain profiling for clonal complex ST-21. This method is suita...

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.

BACKGROUND We evaluated the efficacy of multilocus sequence typing (MLST) for assessing the genetic relationship among Candida albicans isolates from patients with candidemia in a hospital setting. METHODS A total of 45 C. albicans isolates from 21 patients with candidemia were analyzed. The MLST results were compared with results obtained by Southern blot hybridization (C1 fingerprinting) an...

BACKGROUND The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. METHODOLOGY AND FINDINGS We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former g...

The genotyping of pathogens within cystic fibrosis cohorts is an important process, enabling the detection transmissible and clinically-important strains. Traditionally this has been via culture-dependent processes. However, culture-independent investigation respiratory samples becoming more common, with such approaches highlighting limitations culture-based methods. In study we describe applic...

Leptospirosis is a worldwide widespread zoonosis caused by Leptospira genus. We report an acute leptospirosis case in puppy housed at municipal kennel and the subsequent diagnostic investigations carried out on all dogs kennel. Laboratory investigation included mainly microagglutination test, real-time PCR, multi-locus sequence typing (MLST) for Other agents of infection were excluded. The resu...

Abstract Background Campylobacter jejuni (C. ) is a leading cause of acute gastroenteritis in human worldwide. The aim study was to assess the distribution sialylated lipooligosaccharide (LOS) classes and capsular genotypes C. isolated from Iranian children with gastroenteritis. Furthermore, level dnaK gene expression strains selected LOS intended. Moreover, comprehensive MLST-genotypes inclusi...