molecular diagnostic methods have played and continuing to have a critical role in clinical laboratories in recent years. therefore, standardization is an evolutionary process that needs to be upgrade with increasing scientific knowledge, improvement of the instruments and techniques. the aim of this study was to design a quality assurance program in order to have similar conditions for all med...

background: dogs have been previously reported to be reservoirs of leishmania infantum as the etiological agent of human visceral leishmaniasis in iran. we report a case of canine visceral leishmaniasis (vl) caused by l. tropica from the north- west of iran where human visceral leishmaniasis is endemic. methods: the canine vl was initially screened by dipstick rk39 and direct agglutination test...

background: epidemic and endemic cholera is a major public health problem for many   countries. aim of this study was to evaluate ap-pcr for investigation of clonal relatedness among the strains of vibrio cholerae recovered from an out­break occurred in different parts of iran in 2005. methods: the study was conducted during the cholera outbreak occurred in some of provinces in iran in summer 2...

the aim of the study was to determine the resistance patterns of pseudomonas aeruginosa isolates recovered from patients with surgical wounds in hospitals and also to investigate their epidemiological relatedness using molecular typing techniques. twenty pseudomonas sp. isolated from surgical wounds were subjected to antibiotic susceptibility testing by disk diffusion, plasmid profile, sds-page...

results fifty four of 84 (64.2%) slides obtained from patients had positive results microscopically, while 79/84 (94%) of slides had positive results by fta card-nested-pcr. pcr and microscopy showed a sensitivity of 96.4% and 64.2% and specificity of 100% and 100%, respectively. interestingly, leishmania major as causative agent of zoonotic cl was identified in 100% and 90.7% of cl cases from ...

desert truffles, hypogeous pezizales (ascomycota), are difficult to identify due to evolutionary convergence of morphological characters among taxa that share a similar habitat and mode of spore dispersal. in this paper we document the presence of picoa lefebvrei (pat.) maire (=phaeangium lefebvrei) in iran and use phylogenetic analysis of the its and 28s rdna to show that this species belon...

the molecular geometry of complex of adenine with 8 water molecules was calculated with hartree-fock (hf). the standard 6-31g(d) basis set has been employed. the existence of c-h…o hydrogen bonds between the water molecules and the hydrophobic part of nucleobase is stablished. we optimized structures and computed interaction energies of all complexes of adenine with water molecules step by step...

having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. in present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and rapd markers. significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. sheep are intermediate hosts of four sarcocystis species: sarcocystis tenella, sarcocystis gigantea, sarcocystis arieticanis, and sarcocystis medusiformision. the purpose of the study was to determine the molecular identification of the macroscopic and microscopic cysts of sarcocystis in sheep. in...

tomato yellow leaf curl virus (tylcv) is a major tomato virus in tropical and subtropical regions. in this study, 134 accessions of solanum lycopersicum and six accessions of solanum peruvianum were assessed for resistance to an iranian isolate of tylcv. plants were inoculated using whiteflies (bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral dn...