African individuals harbor molecular RH variants, which permit alloantibody formation to high-prevalence Rh antigens after transfusions. Genotyping identifies such RH variants, which are often missed by serologic blood group typing. Comprehensive molecular blood group analysis using 3 genotyping platforms, nucleotide sequencing, and serologic evaluation was performed on a 7-year-old African mal...

Purpose of review Molecular testing methods were introduced to the blood bank and transfusion medicine community more than a decade ago after cloning of the genes made genetic testing for blood groups, that is genotyping, possible. This review summarizes the progress made in the last decade in applying genotyping to prenatal practice and clinical transfusion medicine. Recent findings Assays tha...

Pneumocystis jirovecii pneumonia (PJP) is an opportunistic infection affecting immunocompromised patients. Patients with acute myeloid leukemia (AML) are not considered at high risk of PJP, thus, prophylaxis recommended. Between 2010 and 2020 we retrospectively analyzed 251 AML We performed molecular diagnosis genotyping in 67 bronchoalveolar lavage samples. Eleven cases PJP were diagnosed, a p...

Multilocus sequence typing (MLST) represents the gold standard genotyping method in studies concerning microbial population structure, being particularly helpful in the detection of clonal relatedness. However, its applicability on large-scale genotyping is limited due to the high cost and time spent on the task. The selection of the most informative nucleotide positions simplifies genomic char...

KRAS genotyping is mandatory in metastatic colorectal cancer treatment prior to undertaking antiepidermal growth factor receptor (EGFR) monoclonal antibody therapy. BRAF V600E mutation is often present in colorectal carcinoma with CpG island methylator phenotype and microsatellite instability. Currently, KRAS and BRAF evaluation is based on molecular biology techniques such as SNaPshot or Sange...

Hepatitis C virus (HCV) infection is a major health problem recognized globally. HCV is a common cause of liver fibrosis that may lead to liver cirrhosis or hepatocellular carcinoma. The aim of this study was to estimate the prevalence of HCV infection and genotyping among Egyptian and Saudi Arabian chronic patients using different molecular techniques. HCV RNA viral load was assessed by real-t...

Aim . To improve emergency diagnostic measures and preventive antimicrobial therapy in patients with ixodid tick bites using molecular genetic techniques for identification of Вorrelia spp. Materials Methods Borrelia strains were isolated by inoculation suspension on the BSK-H medium. Molecular screening was performed real-time polymerase chain reaction (PCR) sequencing. In total, we examined 4...

INTRODUCTION Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) RNA in clinical samples have been extensively described. Routine commercial methods for each specific purpose (detection, quantification and genotyping) are also available, all of which are typically based on polymerase chain reaction (PCR) targeting the HCV 5' untranslated region (5'UTR). This st...

Studies involving molecular genetic characterization of silkworms are quite limited. Therefore, with this study, pure and hybrid silkworm lines raised in Turkey was characterized for the first time by microsatellite DNA markers. Samples were collected from 7 different lines, which obtained Provincial Directorate Agriculture Forestry Bursa ones Silkworm Cocoon Association (Kozabirlik). samples e...

In Sudan the leishmania belt extends from Gadarif region from east, where visceral leismaniasis is a predominant form to Darfor region to the west. Cutaneous leishmaniasis is mainly found in northern and western regions beside scattered foci in central part of the country. This study was conducted during the period Sep. 2009 – Sep. 2010 in the Green Valley villages that situated in Rashad regio...