Cultures of spleen cells from immunized mice were stimulated in vitro by soluble preparations of purified foot-and-mouth disease virus. Virus-specific antibody, as detected by an enzyme-linked immunosorbent assay, was produced by immune spleen cells but not by normal, nonimmune cells. The optimal specific response was obtained with 1 microgram of virus per ml of culture; as the virus concentrat...

foot -and- mouth disease (fmd) is one of the most important virus disease in farm animals. types o, a and asia1 fmd virus have been endemic in iran. in these study, samples from suspected livestock were analyzed by rt-pcr experiment. the number of 702 nucleotides determined at 1d -2b region of type a strain isolated from khorasan razavi province sequenced and compared with that of other reporte...

The complete genome of a foot-and-mouth disease type O virus originating from an epidemic in Greece in 1994 is reported. This virus belongs to the Middle East-South Asia topotype.

Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic...

Lethal mutagenesis is an antiviral strategy consisting of virus extinction associated with enhanced mutagenesis. The use of non-mutagenic antiviral inhibitors has faced the problem of selection of inhibitor-resistant virus mutants. Quasispecies dynamics predicts, and clinical results have confirmed, that combination therapy has an advantage over monotherapy to delay or prevent selection of inhi...

BACKGROUND Clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. A cheaper and easy method for differential diagnosis is important in control of this disease in endemic region. METHODS A duplex PCR assay was developed for the specific differential detection of Goatpox virus (GTPV) and Sheeppox virus (SPPV), using two sets of primers based on viral ...

Foot-and-mouth disease appeared as devastating storms that swept UK and Taiwan in recent epidemics. Many observations attributed these disasters to the extensive countrywide movements of animals, and the intensive nature of the livestock industry. However, there is no mathematical model incorporating these two factors in its framework. In this project, we combined formal analytical models, Arti...

Definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. It is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. Due to restrictions of time and sample size, once laboratory tests have ruled out foot...