The analysis of chromatin structure is essential for the understanding of transcriptional regulation in eukaryotes. Here we describe methidiumpropyl-EDTA sequencing (MPE-seq), a method for the genome-wide characterization of chromatin that involves the digestion of nuclei withMPE-Fe(II) followed by massively parallel sequencing. Like micrococcal nuclease (MNase), MPE-Fe(II) preferentially cleav...

OBJECTIVE Oral once-daily dual-release hydrocortisone (DR-HC) replacement therapy was developed to provide a cortisol exposure-time profile that closely resembles the physiological cortisol profile. This study aimed to characterize single-dose pharmacokinetics (PK) of DR-HC 5-20mg and assess intrasubject variability. METHODS Thirty-one healthy Japanese or non-Hispanic Caucasian volunteers age...

1 Chinese malignant pleural effusion (MPE) diagnosis and treatment expert group fi rst proposed the consensus of diagnosis and treatment of malignant pleural effusion in 2013, which was published in 2014. This plays a positive role in regulating, promoting and facilitating clinical and research work of MPE. Though problems were frequently encountered in diagnosis, differential diagnosis and tre...

BACKGROUND Pharmacokinetic/pharmacodynamic (PK/PD) parameters of neuromuscular blocking agents (NMBAs) are generally assumed to be dose-independent. To our knowledge, there are very few clinical reports where the PK/PD parameters of a NMBA were derived separately for each dose group during a formal dose-ranging study. The primary objective of this study was to challenge a potential dose-depende...

Accumulating evidence shows that an imbalance in regulatory T cells (Tregs)/T helper IL-17-producing cells (Th17) exists in malignant pleural effusion (MPE). However, the cause of this phenomenon in MPE and the underlying mechanism remain uncertain. The percentages of Tregs and Th17 cells in MPE and parapneumonic effusion (PPE) were determined by flow cytometry. Their specific transcription fac...

Mycoplasma infection has been reported in immunocompromised cancer patients; nevertheless, it is not clear if persistent Mycoplasma infection could facilitate the proliferation of cancer cells in immunocompromised organisms. The aim of this study was to examine the relationship between persistent Mycoplasma infection and malignant transformation in an immunodeficient host model. Immunodeficient...

This paper describes a novel instrument used for the analysis of full-field throughthickness stress distributions using the theory of magnetophotoelasticity (MPE) developed by Aben [1] and developed by Clarke et al. [2, 3]. MPE is an experimental stress analysis technique that involves the application of a magnetic field parallel to an electromagnetic wave propagating through a birefringent mod...

Background. There are no available literatures on massive pleural effusions (MPE) in our country. Aim. To determine the aetiology of MPE and compare the mortality rate between malignant and nonmalignant MPE in adult Nigerians. Methods. A prospective study of all the patients diagnosed with nontraumatic pleural fluid collections for one year in two tertiary federal hospitals in Southern Nigeria....

This paper addresses the use of discriminative training criteria for Speaker Adaptive Training (SAT), where both the transform generation and model parameter estimation are estimated using the Minimum Phone Error (MPE) criterion. In a similar fashion to the use of I-smoothing for standard MPE training, a smoothing technique is introduced to avoid over-training when optimizing MPEbased feature-s...