Bovine respiratory syncytial virus (BRSV) is a pneumovirus in the family paramyxoviridae, is an important cause of acute respiratory disease in postweaning calves and feedlot cattle. The real-time reverse transcriptase PCR protocols were developed to detect BRSV infection in infected animals. The sensitivity of RT-PCR protocols based on fusion gene were evaluated using different Mastermixes suc...

The citrus systemic diseases, including Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), tristeza virus (CTV)), tatter leaf (CTLV)), and exocortis viroid (CEVd)), are threats to production in Taiwan. Reliable diagnostic methods important for the management of these diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay...

List of non-standard abbreviations ATSDR Agency for Toxic Substances and Disease Registry GSK-3β Glycogen synthase kinase-3β Kim-1 Kidney injury molecule-1 RT-PCR Reverse transcriptase polymerase chain reaction

A multiplex reverse transcription polymerase chain reaction (RT-PCR) was developed and optimized to simultaneously detect 3 subtypes (H5, H7 and H9) of avian influenza virus type A. The developed method was specific to amplify only the HA gene of H5, H7 and H9 of Influenza type A and no amplification with other subtypes of AIV and other avian infectious viruses such as Newcastle disease virus, ...

Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...

Wild bird carcasses were collected through passive monitoring of wild bird mortality. In necropsy, brain samples were taken from dead wild birds in 2010, 2011 and 2012. Samples were used for detection of West Nile virus by reverse transcriptase-polymerase chain reaction (RT-PCR). No WNV nucleic acid was detected by RT-PCR. In the light of the data presented there is no evidence of wild bird mor...

AIM The aim of this study was to develop multiplex-PCR assays for the detection of circulating tumor cells in peripheral blood and urine samples of patients with bladder cancer. MATERIALS AND METHODS Peripheral blood and urine samples were collected from 208 patients (169 patients and 39 healthy volunteers). After RNA extraction and cDNA synthesis, the samples were analyzed for the expression...