The citrus systemic diseases, including Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), tristeza virus (CTV)), tatter leaf (CTLV)), and exocortis viroid (CEVd)), are threats to production in Taiwan. Reliable diagnostic methods important for the management of these diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay...

BACKGROUND AND OBJECTIVES HIV-1 and HCV infections are life threatening problems in patients who receive blood products. Serological methods have proven useful in detecting these infections, but there are setbacks that make it challenging to detect these infectious agents. By the advent of Nucleic Acid Testing (NAT) methods, especially in multiplex format, more precise detection is possible. ...

Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus, rotavirus, norovirus genogroup I (GI) and GII, and astrovirus. We describe a...

Three serotypes of foot-and-mouth disease virus (FMDV) including A, Asia1 and O, have been detected in Iran. Following the mass vaccination program which was implemented in all parts of the country in 2002, the number of outbreaks has been significantly reduced. Therefore, rapid detection of FMDV and its serotypes in clinical samples is essential for control of new outbreaks. In this study a re...

Garlic generally becomes coinfected with several types of viruses belonging to the Potyvirus, Carlavirus, and Allexivirus genera. These viruses produce characteristically similar symptoms, they cannot be easily identified by electron microscopy (EM) or immunological detection methods, and they are currently widespread around the world, thereby affecting crop yields and crop quality adversely. F...