background and objectives: tuberculosis (tb) is the oldest known bacterial disease in humans. due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. materials and methods: in this study we used fluorescent amplification–based specific hybridization (flash) pcr to target is6110 for rapid detection of m. tuberculosis (mtb). to investigate the important factors ...

Background: Paratuberculosis and tuberculosis (TB) caused by Mycobacterium avium paratuberculosis (MAP) and Mycobacterium tuberculosis complex (MTC), respectively are economically important, chronic debilitating diseases affecting the dairy herds and are also potential zoonotic threats. Aims: Differential diagnosis of paratuberculosis and TB i...

Mycobacterium marinum, a close relative of Mycobacterium tuberculosis is being used to study mycobacterial pathogenesis. M. marinum causes a systemic tuberculosis-like infection and disease in ectotherms such as frogs and fish. This review describes the development of M. marinum as a model pathogen and the more recent development of genetically tractable model hosts, namely the zebrafish, Droso...

This study examined the role of intranasal vaccination with Mycobacterium tuberculosis antigen85 complex proteins formulated in dimethyldioctadecylammonium bromide against airway Mycobacterium tuberculosis challenge in mice. Intranasal vaccination with antigen85A and antigen85B induced a significantly higher level of interferon-gamma, interleukin-12 and interleukin-4 in cervical lymph nodes tog...

1. Brudey K, Driscoll JR, Rigouts L, Prodinger WM, Gori A, Al-Hajoj SA, et al. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classifi cation, population genetics and epidemiology. BMC Microbiol. 2006;6:23–39. 2. Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Taiwan....

Bovine tuberculosis is an infectious disease that occurs in many species of both domestic and wild animals, as well as those held in captivity. The etiological factor is the acid resistant bacillus (Mycobacterium bovis or Mycobacterium caprae), which is characterized by the major pathogenicity among mycobacteria belonging to the Mycobacterium tuberculosis complex. The material from 8 antelopes ...

For the optimal recovery of Mycobacterium tuberculosis from the BACTEC™ Mycobacterium Growth Indicator Tube 960™ system, an incubation period of 42-56 days is recommended by the manufacturer. Due to logistical reasons, it is common practice to follow an incubation period of 42 days. We undertook a retrospective study to document positive Mycobacterium Growth Indicator Tube cultures beyond the 4...

background pe5 is a member of the pe protein family whose precise function is yet to be understood. there are about 100 members of the pe family proteins in mycobacterium tuberculosis. these glycine- and alanine-rich proteins consist of proline-glutamate motifs at their n-termini and may be implicated in pathogenesis of the bacilli. objectives we aimed to clone and over-express the rv0285 codin...

Most control programs for bovine tuberculosis include reporting to both veterinary and public health authorities, and measures to prevent transmission from animals to humans. On the other hand, reporting of human cases to veterinary authorities is rare, unless an animal source is suspected. Exchange of data and strategic discussions between veterinary and public health authorities would strengt...

Mycobacterium marinum, a causative agent of fish tuberculosis, is one of the most closely related Mycobacterium species (outside the M. tuberculosis complex) to M. tuberculosis, the etiologic agent of human tuberculosis. Signature-tagged mutagenesis was used to identify genes of M. marinum required for in vivo survival in a goldfish model of mycobacterial pathogenesis. Screening the first 1008 ...