نتایج جستجو برای: nanobodies

تعداد نتایج: 541  

Journal: :World Chinese Journal of Digestology 2015

Journal: :Frontiers in Immunology 2023

Introduction T cell Ig and ITIM domain receptor (TIGIT) is a next-generation immune checkpoint predominantly expressed on activated cells NK cells, exhibiting an unfavorable prognostic association with various malignancies. Despite the emergence of multiple TIGIT-blocking agents entering clinical trials, only fraction patients responded positively to anti-TIGIT therapy. Consequently, urgent dem...

2017
Ronan Crépin David Gentien Angeline Duché Audrey Rapinat Cecile Reyes Fariba Némati Gérald Massonnet Didier Decaudin Selma Djender Sandrine Moutel Klervi Desrumeaux Nathalie Cassoux Sophie Piperno-Neumann Sebastian Amigorena Franck Perez Sergio Roman

Ronan Crépin, David Gentien, Angeline Duché, Audrey Rapinat, Cecile Reyes, Fariba Némati, Gérald Massonnet, Didier Decaudin, Selma Djender, Sandrine Moutel, Klervi Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman Roman, Ario de Marco (2017 Feb 1) Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal...

2015
Yeong Ju Shin Seung Kyun Park Yoo Jung Jung Ye Na Kim Ki Sung Kim Ok Kyu Park Seung-Hae Kwon Sung Ho Jeon Le A. Trinh Scott E. Fraser Yun Kee Byung Joon Hwang

Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish embryos. This approach is easily modif...

2017
Pieter Deschaght Ana Paula Vintém Marc Logghe Miguel Conde David Felix Rob Mensink Juliana Gonçalves Jorn Audiens Yanik Bruynooghe Rita Figueiredo Diana Ramos Robbe Tanghe Daniela Teixeira Liesbeth Van de Ven Catelijne Stortelers Bruno Dombrecht

Next-generation sequencing (NGS) has been applied successfully to the field of therapeutic antibody discovery, often outperforming conventional screening campaigns which tend to identify only the more abundant selective antibody sequences. We used NGS to mine the functional nanobody repertoire from a phage-displayed camelid immune library directed to the recepteur d'origine nantais (RON) recept...

2014
Linda De Vooght Guy Caljon Karin De Ridder Jan Van Den Abbeele

BACKGROUND Sodalis glossinidius, a vertically transmitted microbial symbiont of the tsetse fly, is currently considered as a potential delivery system for anti-trypanosomal components that reduce or eliminate the capability of the tsetse fly host to transmit parasitic trypanosomes, an approach also known as paratransgenesis. An essential step in developing paratransgenic tsetse is the stable co...

2016
Jinwoo Leem James Dunbar Guy Georges Jiye Shi Charlotte M. Deane

Computational modeling of antibody structures plays a critical role in therapeutic antibody design. Several antibody modeling pipelines exist, but no freely available methods currently model nanobodies, provide estimates of expected model accuracy, or highlight potential issues with the antibody's experimental development. Here, we describe our automated antibody modeling pipeline, ABodyBuilder...

2015
Jonas Helma M. Cristina Cardoso Serge Muyldermans Heinrich Leonhardt

Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, includ...

Journal: :Methods and applications in fluorescence 2015
David Albrecht Christian M Winterflood Helge Ewers

Single particle tracking is a powerful tool to investigate the function of biological molecules by following their motion in space. However, the simultaneous tracking of two different species of molecules is still difficult to realize without compromising the length or density of trajectories, the localization accuracy or the simplicity of the assay. Here, we demonstrate a simple dual color sin...

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