Because thrombin-induced inflammation is partially mast cell-dependent and involves proteinase-activated receptors (PARs), we hypothesized that mast cells express PAR and can be stimulated with PAR-activating peptides (PAR-AP). We demonstrated that rat peritoneal mast cells expressed PAR-1 and PAR-2 mRNA, and that PAR-2AP (tc-LIGRLO-NH(2), 1 microm) induced 64.2 +/- 4.4% specific beta-hexosamin...

Abstract We developed a novel method for production of no-carrier-added (n.c.a.) [ 188, 189, 191 Pt]Pt II Cl 4 2− from an Ir target material, and then synthesized n.c.a. [*Pt]cis-[Pt 2 (NH 3 ) ] ([*Pt]cisplatin) [*Pt]Pt . was prepared as synthetic precursor *Pt complex by combination resin extraction anion-exchange chromatography after the selective reduction IV 6 with ascorbic acid. The ligand...

Of six strains of Ruminococcus bromii studied, five grew in a minimal chemically defined medium containing minerals, NH(4) (+) as nitrogen source, sulfide or sulfate as sulfur source, fructose as energy and carbon source, isobutyrate or 2-methylbutyrate and carbonic acid-bicarbonate as additional carbon sources, and the vitamins biotin, riboflavin, pyridoxine, vitamin B(12) (replaced by L-methi...

The present study investigated the role of ammonia as a trigger for hatching, mechanisms of ammonia detoxification and the localization of urea cycle enzymes in the early life stages of freshwater rainbow trout (Oncorhynchus mykiss). The key urea cycle enzyme carbamoyl phosphate synthetase III was found exclusively in the embryonic body (non-hepatic tissues); related enzymes were distributed be...

Micrometeorological measurements of sizesegregated particle number fluxes above Dutch heathlands and forests have repeatedly shown simultaneous apparent emission of particles with a diameter (Dp)<0.18μm and deposition of larger particles when measured with optical particle counters. In order to assess whether this observation may be explained by the equilibrium reaction of ammonia (NH3), nitric...

Nonionic diffusion and diffusion equilibrium of ammonia have been generally accepted as the mechanism of urinary ammonium excretion. However, these characteristics have not been examined directly in vitro. In the present studies, nonionic diffusion and diffusion equilibrium of ammonia were examined in rabbit cortical collecting tubules perfused in vitro. Collected fluid ammonium and pH were mea...

The kidney plays a major role in acid-base homeostasis by adapting the excretion of acid equivalents to dietary intake and metabolism. Urinary acid excretion is mediated by the secretion of protons and titratable acids, particularly ammonia. NH(3) is synthesized in proximal tubule cells from glutamine taken up via specific amino acid transporters. We tested whether kidney amino acid transporter...

Методами спектроскопии ИК- и ЯМР Н С с использованием теоретических расчетов (программы ChemDrawUltra, Marvin, ACD/Labs) изучены спектральные характеристики L-цистеинсульфоновой кислоты (L-cysSOH) в растворах виде частично протонированной формы (SO)CHCH(NH)COO-(рН=3) депротонированного иона(SO-)CHCH(NH)COO-(рН=10). Установлено, что отличие от спектров кислоты, спектрах не наблюдается сильного с...