Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...

Glucose g-phosphate dehydrogenase was first described in yeast by Warburg and Christian (l), who showed that nicotinamide adenine dinucleotide phosphate was the specific coenzyme. The enzyme was later prepared from liver and again shown to be specific for the same coenzyme (2). Subsequent preparations (see Cheldelin (3)) from various microbial sources have included two which react with both nic...

Administration of a single dose of aflatoxin B1 (AFB1) (6 mg/kg) to Sprague-Dawley rats results in a high level of modification of hepatic mitochondrial DNA (2.1 nmol of AFB1 adducts per mumol DNA-phosphate) and long-term inhibition of mitochondrial transcription and translation activities (N. Bhat et al., Cancer Res., 42: 1876-1880, 1982). Similar doses of AFB1 given to ICR mice and Syrian gol...

Chronic granulomatous disease (CGD) is an extremely rare genetically heterogeneous disorder characterized by serious life-threatening infections. CGD caused a defect of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system. The increased inflammation and granuloma formation secondary to recurrent typically involves lungs, liver, lymph nodes. Most patients with are diagnosed in ...

To develop a nicotinamide-independent single flavoenzyme system for the asymmetric bioreduction of C=C bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. Six highly potent, cheap, and commercially available co-substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even super...

The metabolism of glucose was examined in several clinical isolates of Neisseria gonorrhoeae. Radiorespirometric studies revealed that growing cells metabolized glucose by a combination on the Entner-Doudoroff and pentose phosphate pathways. A portion of the glyceraldehyde-3-phosphate formed via the Entner-Doudoroff pathway was recycled by conversion to glucose-6-phosphate. Subsequent catabolis...