Measuring the nuclear magnetic resonance spectra of low-gamma heteronuclei such as 15N constitutes an important analytical tool for the characterization of molecular structure and dynamics. The reduced resonance frequencies and magnetic moments of these heteronuclei, however, make the sensitivity of this kind of spectroscopy inherently lower than that of comparable H NMR observations. A well-kn...

9.1 Saturation Transfer Difference NMR Principle ......... " 149 9.2 STD-NMR for Characterizing Ligand Binding to Membrane Integrated Proteins. . . . . . . . . . . . . . . . . . .. 153 9.3 Epitope Mapping with STD-NMR . . . . . . . . . . . . . . . . . .. 157 9.4 Group Epitope Mapping with STD-NMR .............. l57 9.5 Binding Constants from STD-NMR Experiments ......... 160 9.6 STD-NMR Analysi...

We report here a biochemical and structural characterization of domain 2 of the nonstructural 5A protein (NS5A) from the JFH1 Hepatitis C virus strain and its interactions with cyclophilins A and B (CypA and CypB). Gel filtration chromatography, circular dichroism spectroscopy, and finally NMR spectroscopy all indicate the natively unfolded nature of this NS5A-D2 domain. Because mutations in th...

A simple and robust solvent suppression technique that enables acquisition of high-quality 1D 1 H nuclear magnetic resonance (NMR) spectra of alcoholic beverages on cryoprobe instruments was developed and applied to acquire NMR spectra of Scotch Whisky. The method uses 3 channels to suppress signals of water and ethanol, including those of 13 C satellites of ethanol. It is executed in automatio...

Professor Jonathan Parr By submitting this essay, I attest that it is my own work, completed in accordance with University regulations.—Andrew Yang An Introduction to Nuclear Magnetic Resonance Spectroscopy by Andrew Yang Nuclear Magnetic Resonance Spectroscopy, or NMR Spectroscopy, is a powerful nondestructive research technique that uses the magnetic properties of nuclei to investigate the co...

regioselective synthesis of highly functionalized pyranopyrazoles was achieved in excellent yield from phenyl pyrazolone, substituted aromatic aldehyde with nitroketene-n,s-acetal in the presence of indium trichloride as a versatile catalyst under reflux condition in ethanol-water mixture. all reactions preceeded within a short period of time with excellent purity. all of the synthesized compou...

in this paper the synthesis of some new polyaromatic monocyclic β-lactams has been reported. these cycloadducts have been synthesized by the [2+2] ketene-imine cycloaddition reaction known as the staudinger reaction. all of the products have been fully characterized by ir, 1h-nmr, 13c-nmr, mass spectroscopy and elemental analyses. the x-ray crystal structure of a cis β-lactam has been also pres...