Background: Transgenic mice are being considered as invaluable tool in biological sciences towards comprehension of the cause of the genetic diseases. Manipulated embryonic stem (ES) cells are used to produce loss-of-function mutant mice. Microinjection of manipulated ES cells into blastocoel cavity, and morula fusion are the two main techniques in producing transgenic mice. So...

Regenerative medicine using stem cells has progressed significantly over the last decade. Plastic surgeons historically have used tissues of human being to restore various defect sites and utilized a single cell lines for the tissue regeneration. The cell sources (autologous or allogeneic), cell types (embryonic stem cell or adult stem cell), and source of tissues (bone marrow, muscle, adipose,...

Objective To establish C57BL/6J embryonic stem (ES) cell lines with potential germline contribution Methods ES cells were isolated from blastocyst inner cell mass of C57BL/6J mice, and cultured for 15 passages, and then injected into blastococels of ICR mice blastocysts to establish chimeric mice. Results Three ES cell lines (mC57ES1,mC57ES3, mC57ES7) derived from the inner cell mass of C57BL/6...

Our Department of Experimental Embryology originated from The Laboratory of Embryo Biotechnology, which was organized and directed by Dr. Maria Czlonkowska until her premature death in 1991. Proving successful international transfer of frozen equine embryos and generation of an embryonic sheep-goat chimaera surviving ten years were outstanding achievements of her term. In the 1990s, we produced...

embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...

The Royal College of Surgeons (RCS) rat is an animal model for retinal degeneration such as the age-related macular degeneration. The RCS rat undergoes a progressive retinal degeneration during the early postnatal period. A potential treatment to prevent this retinal degeneration is the transplantation into the subretinal space of cells that would replace functions of the degenerating retinal p...

Stem cells are undifferentiated cells that have the ability of proliferation, regeneration, conversion to differentiated cells and tissue production. Regeneration means that these cell shave the ability of asymmetric division, which one of the resulting cells remains as stem cell while another cell, which is called daughter cell, becomes one cell of germ layer. Stem cells may remain inactive fo...

Stem cells are undifferentiated cells that have the ability of proliferation, regeneration, conversion to differentiated cells and tissue production. Regeneration means that these cell shave the ability of asymmetric division, which one of the resulting cells remains as stem cell while another cell, which is called daughter cell, becomes one cell of germ layer. Stem cells may remain inactive fo...

BACKGROUND/AIM Obtaining human embryonic stem cell lines has so far involved destroying the embryos. This has given rise to ethical concerns and is not permitted in most countries. This investigation tested whether removing multiple cells from blastocysts might allow continued embryonic development. MATERIALS AND METHODS A total of 40 blastocysts from a black mouse strain were biopsied. The m...