نتایج جستجو برای: non denaturing solubilization
تعداد نتایج: 1325356 فیلتر نتایج به سال:
This study aimed to evaluate the impact of genetically modified (GM) wheat with introduced pm3b mildew resistance transgene, on two types of root-colonizing microorganisms, namely pseudomonads and arbuscular mycorrhizal fungi (AMF). Our investigations were carried out in field trials over three field seasons and at two locations. Serial dilution in selective King's B medium and microscopy were ...
The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and steps. Denaturing compounds are used to reduce temperature, which keeps the proper morphology of preparation. Formamide is most commonly reagent lower melting temperature. substitution toxic formamide a non-toxic ethylene carbonate at 20 % 50 concentration mixture helpe...
Laccase is a bio catalytic agent and multi-copper enzyme containing oxidases that are potentially great for oxidizing large number of phenolic non-phenolic compounds. However, drawbacks do arise when laccase use in scale; low stability, high production cost, non-reusability, sensitive towards denaturing poor storage ability free enzymes. These problems lead to the progress immobilization order ...
Solubilization of the human erythrocyte membrane by seven detergents is described. Components released into the supernatant or retained in the residue were identified by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Two non-ionic detergents exhibiting little u.v. absorption were more efficient than u.v.-absorbing Triton X-100. Evidence is presented of an interchange between protei...
the effect of triton x-100, na cholate and tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of salmonella typhimurium was studied. the detergents were used in various concentrations (1.6 to 64 mm) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. results showed that no inne...
Chemical changes and shelf-life of common carp (Cyprinus carpio) surimi prepared by conventional washing method and solubilization by acid and alkali processes were investigated during 5 months of storage at -18 °C. Results showed that surimi produced with acid and alkaline solubilization is significantly higher yield than the conventional surimi. Protein solubilization by using acid and alkali...
Potential interactions between membrane components of rat brain synaptic vesicles were analyzed by detergent solubilization followed by size fractionation or immunoprecipitation. The behavior of six synaptic vesicle membrane proteins as well as a plasma membrane protein was monitored by Western blotting. Solubilization of synaptic vesicle membranes in CHAPS resulted in the recovery of a large p...
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