Isotopic and nuclear techniques play an important role in food and agriculture, health and industry. In the agricultural sciences and food technology sectors, recent research has elucidated the new potential application of radiation for microbial decontamination, food preservation, improve the nutritional quality of  foods  and effectively utilise its potential as human food or animal feed. The...

Isotopic and nuclear techniques play an important role in food and agriculture, health and industry. In the agricultural sciences and food technology sectors, recent research has elucidated the new potential application of radiation for microbial decontamination, food preservation, improve the nutritional quality of  foods  and effectively utilise its potential as human food or animal feed. The...

  Solitary pulmonary nodule (SPN) is a frequent finding on the chest x-ray and computed tomography. Nuclear medicine techniques play an important role in the diagnosis and management of SPN. In the current review, we briefly will explain the different nuclear medicine modalities in this regard including positron emission tomography (PET) using 18-F-FDG, and 11-C-Methionine, and single photon em...

Somatic cell nuclear transfer is a complex and intricate procedure with a low success rate. Despite advances in embryo culture and the production of specialized tools and equipment success rate has remained poor. The procedure remains basically the same despite technical innovations but is now easier to learn and requires less technical expertise. Oocytes now come from an in vitro system, which...

in this research, the microstructural of low molecular weight 1,2-polybutadiene (1,2-pbd) was conducted by 1h and 13c nuclear magnetic resonance spectroscopy (nmr) to determine the isomeric contents of 1,4-cis, 1,4-trans and 1,2-vinyl in 1,2-pbd polymer structures. number average molecular weight for low molecular weight 1,2-pbd was measured by nmr techniques and the results were compared with ...

The first transgenic pigs were generated for agricultural purposes about three decades ago. Since then, the micromanipulation techniques of pig oocytes and embryos expanded from pronuclear injection of foreign DNA to somatic cell nuclear transfer, intracytoplasmic sperm injection-mediated gene transfer, lentiviral transduction, and cytoplasmic injection. Mechanistically, the passive transgenesi...

Nuclear reprogramming toward pluripotency has been now achieved either in vivo by somatic cell nuclear transfer into the ooplasm of an enucleated egg, or in vitro by three different approaches, namely cell fusion, treatment with cell extracts and more recently, forced expression of a reduced set of defined transcription factors. This last technique has expanded our view of genome plasticity wit...

OBJECTIVES The Streptomyces phage phiC31 integrase offers a sequence-specific method of transgenesis with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose of in vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embry...

BACKGROUND Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for biomedical research. However, the efficiency of this approach is crucially dependent on the source of nuclear donor cells. In this study, we evaluate the potential of primary porcine kidney cells (PKCs) as cell source for SCNT, including their prolif...