The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucleic acids by hybridization. Two different approaches are described. A sequence specific method and a generic method. The sequence specific method requires sequence information on the target and synthesis of a dedicated PNA. It can be used to selectively purify the nucleic acid containing the targ...

A virus, inactivated by ultraviolet light, was used to fuse together cells from different species of vertebrate, and the resulting heterokaryons were examined by autoradiographic and cytological techniques. Heterokaryons could be made with both differentiated and undifferentiated cells: HeLa and Ehrlich ascites cells were studied as examples of undifferentiated cells; rabbit macrophages, rat ly...

T-even bacteriophage-tolerant mutants are strains of Escherichia coli which can adsorb T-even phages but cannot support the growth of infective virus. Under some conditions, the infected cells are not killed. Mutant cells infected by phage T6 are able to carry out several metabolic functions associated with normal virus development, including arrest of bacterial nucleic acid and protein synthes...

The rate of synthesis of stable nucleic acid was measured during the division cycle in slowly growing cultures of Escherichia coli B/r. The experimental patterns of synthesis were consistent with a discontinuous mode of chromosome replication and an exponentially increasing rate of stable RNA synthesis. The rates of tRNA and rRNA synthesis relative to the rate of total stable RNA synthesis were...

In the light of suggestions in the literature linking the car cinogenic action of urethan (ethyl carbamate) with the inhibi tion of an enzymatic step in nucleic acid metabolism (par ticularly pyrimidine synthesis), a series of enzymes has been assayed in vitro at concentrations of urethan which are supralethal in mice. These enzymes were: ornithine transcarbamylase; the enzymes leading to the s...

Phosphonoacetic acid specifically inhibited human cytomegalovirus DNA synthesis in virus-infected human fibroblasts as detected by virus-specific nucleic acid hybridization. Inhibition was reversible; viral DNA synthesis resumed upon the removal of the drug. The compound partially inhibited DNA synthesis of host cells in the log phase of growth but had little effect on confluent cells. Studies ...