The virion-associated RNA polymerase and the structural nucleoprotein of influenza A virus were separated by sodium dodecyl sulfate/PAGE, electroblotted to a polyvinylidine membrane, and eluted with good recovery from the membrane. After renaturation by incubating with Escherichia coli thioredoxin, these proteins were active in a reconstituted in vitro transcription reaction with purified genom...

Escherichia coli RecA protein, in the presence of ATP or its analog adenosine 5'-[gamma-thio]triphosphate, polymerizes on single-stranded DNA to form nucleoprotein filaments that can then bind to homologous sequences on duplex DNA. The three-stranded joint molecule formed as a result of this binding event is a key intermediate in general recombination. We have used affinity cleavage to examine ...

Studies originating with plant viruses led to the concept that plasmodesmata potentiate the cell-to-cell trafficking of viral and endogenous proteins and nucleoprotein complexes. In this article, we develop the theme that, at the tissue/organ level, cell-to-cell trafficking of information molecules enables noncell-autonomous control over a range of processes, whereas at the organismal level, th...

Much progress has recently been made in the study of the ribonucleoprotein particles associated with the microsomal fraction of cell homogenates. The shape and size of these particles as well as their distribution and organization in animal tissues have been studied with the electron microscope (1-4). They appear as round particles with diameters of 100 to 300 A in OsO4-fixed preparations of bo...

viral infections are the cause of great economic losses in the poultry industry. development of appropriate reagents and serological diagnostic kits will help to control these infections. the aim of this study is to prepare a peroxidase labeled anti-chicken igg using a mab (5b8) against chicken igg, for detection of chicken antibodies in elisa. hybridoma cells producing the mab 5b8 were culture...

Copyright: © 2015 Reiche et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This study describes the protective efficacy of a novel influenza plasmid DNA vaccine in ferret challenge model. The rationally designed polyvalent encodes haemagglutinin and neuraminidase proteins derived from less glycosylated pandemic H1N1 (2009) H3N2 (1968) virus strains as well nucleoprotein (NP) matrix (M1 M2) different (1918) strain. Needle-free intradermal immunisation with protected fe...