On base of stock-breeding and poultry wastes the cheap nutrient media for cultivation and production of biomass of phototrophic bacteria have been developed. Cultivation of purple nonsulfur bacteria Rhodobacter sphaeroides together with Bacillus megaterium in mixed culture has been succeeded in high biomass yield. The obtained biomass was distinguished with rich contents of biologically active ...

In cross-streak tests on nutrient agar containing lactate, acetate, or citrate, the growth of certain enteric bacteria was inhibited by themselves or by Vibrio cholerae, or by both. This mimicked the activity of bacteriocins, but the phenomenon was attributable to the accumulation of carbonate and increased alkalinity of the media.

Forty-four novel strains of Gammaproteobacteria were cultivated from coastal and pelagic regions of the Pacific Ocean using high-throughput culturing methods that rely on dilution to extinction in very low nutrient media. Phylogenetic analysis showed that the isolates fell into five rRNA clades, all of which contained rRNA gene sequences reported previously from seawater environmental gene clon...

The rhizobial bacteria can be readily grown on a variety of media containing complex nutrient extracts of microbial, mineral, or plant origin, but growth on nonsupplemented carbohydrate-inorganic salts media is poor for most species. Only a minor part of the extensive research devoted to Rhizobium has dealt with the minimum requirements for cultural growth (Wilson, 1940; Wilson and Wilson, 1942...

The effects on porcine arterial structure and permeability of a 4-hour in vitro incubation at 37 degrees C in eight different blood-derived and synthetic nutrient media were examined. Changes in arterial permeability were inferred from the normalized, 1-hour, pressurized, transendothelial uptake (M/c0 cm) of porcine 125I-albumin in 60 porcine aortic tissue preparations using an organ-support sy...

Current industry practices for large-scale mammalian cell cultures typically employ a standard platform fed-batch process with fixed volume bolus feeding. Although widely used, these processes are unable to respond to actual nutrient consumption demands from the culture, which can result in accumulation of by-products and depletion of certain nutrients. This work demonstrates the application of...

Studies have shown that mesenchymal stem/stromal cells (MSCs) from bone marrow are involved in the growth and metastasis of solid tumors but the mechanism remains unclear in osteosarcoma (OS). Previous studies have raised the possibility that OS cells may receive support from associated MSCs in the nutrient deprived core of the tumors through the release of supportive macromolecules and growth ...

Phytoremediation depends on the ability of plants to tolerate and assimilate contaminants. This research characterized the interaction between several ornamental plant species and the fungicidal active ingredient, metalaxyl [N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alanine methyl ester]. Species evaluated included sweetflag (Acorus gramineus Sol. ex Aiton), canna (Canna hybrida L. 'Yellow King H...

Neurospora crassa conidia incubating in buffer at low water activities (a(w)) release a germination-essential component as well as 260-nm absorbing and ninhydrin-positive materials, regardless of whether an electrolyte or non-electrolyte is used to reduce a(w). Chloroform and antibiotics known to increase cell-membrane permeability have a similar effect. This suggests that membrane damage occur...

Plant nutrition studies frequently require nearly complete elimination of trace metals from the nutrient media in which the plants are to be grown. Conventional methods of purification by recrystallization and precipitation have been used for this purpose in some studies. These have proved more difficult and less effective than the adsorption technique developed by STEINBERG (11) and improved b...