Outer membrane vesicles (OMVs) of the periodontopathic bacterium Porphyromonas gingivalis contain a wide range of virulence factors including lipopolysaccharide (LPS), fimbriae and gingipains. We have recently reported strong immunogenicity of OMVs using an intranasal vaccine mouse model. In the present study, we performed sub-immunoproteome analysis of OMV-immunized mouse serum samples from si...

Bacteria use a variety of secreted virulence factors to manipulate host cells, thereby causing significant morbidity and mortality. We report a mechanism for the long-distance delivery of multiple bacterial virulence factors, simultaneously and directly into the host cell cytoplasm, thus obviating the need for direct interaction of the pathogen with the host cell to cause cytotoxicity. We show ...

Automation is an essential component in today’s semiconductor manufacturing As factories migrate to 300 mm technology, automated handling becomes increasingly important for variety of ergonomic, safety, and yield considerations. Traditional semiconductor AMHS systems, such as the Overhead Monorail Vehicles (OMV) or Overhead Hoist, can be overly expensive. Cost projections for a 300mm inter/intr...

Neisseria meningitidis and Bordetella pertussis are Gram-negative bacterial pathogens that can cause serious diseases in humans. N. meningitidis outer membrane vesicle (OMV) vaccines and whole cell pertussis vaccines have been successfully used in humans to control infections with these pathogens. The mechanisms behind their effectiveness are poorly defined. Here we investigated the role of Tol...

In this work, we introduce a microscopic traffic flow model called Scalar Capacity Model (SCM) which can be used to study the formation of on an airway link for autonomous Unmanned Aerial Vehicles (UAVs) as well ground vehicles road. Given 3D trajectory UAV flights (as opposed 2D vehicles), main novelty in our is eliminate commonly notion lanes and replace it with density capacity flow, but suc...

Burkholderia pseudomallei (Bps)is the causative agent of melioidosis and is endemic in regions of northern Australia and Southeast Asia. Bps is inherently resistant to multiple antibiotics and is considered a potential biological warfare agent by the U.S. DHHS. Therefore, effective vaccines are necessary to prevent natural infection and to safeguard against biological attack with this organism....

Bacterial outer membrane vesicles (OMVs) are nano-sized compartments consisting of a lipid bilayer that encapsulates periplasm-derived, luminal content. OMVs, which pinch off of Gram-negative bacteria, are now recognized as a generalized secretion pathway which provides a means to transfer cargo to other bacterial cells as well as eukaryotic cells. Compared with other secretion systems, OMVs ca...

This paper discusses the design challenges encountered when building an ontology repository for an application framework devoted to assistive technologies that can be browsed and queried in a highly heterogeneous and expressive way. As a main vehicle to achieve this goal we propose to use metadata and meta-reasoning. We analyze how metadata can be used in the context of open repositories of ont...

tomation is an essential component in today’s iconductor manufacturing As factories migrate to 300 technology, automated handling becomes increasingly portant for variety of ergonomic, safety, and yield nsiderations. Traditional semiconductor AMHS systems, h as the Overhead Monorail Vehicles (OMV) or erhead Hoist, can be overly expensive. Cost projections a 300mm inter/intrabay AMHS installatio...