Heterozygosity is the major constraint in perennial fruit crop like mango for regular bearing breeding. Majority of popular varieties have irregular habit. Many external and internal factors affect habit crops. Among factors, level carbohydrate reserves phytohormones plays a role on crops apple, citrus, mango, litchi etc., Therefore, present research work aimed to study metabolism pathways geno...

a specific polymerase chain reaction (pcr) method was applied for identification of bovine (bos taurus), ovine (ovis aries) and caprine (capra hircus) pure and binary mixtures of raw and heat-processed meats. these meats are used in food industry products and/or for direct consumption of consumers. the mitochondrial dna was amplified as a template in a pcr reaction by use of specific primers re...

This paper delivers population genetic data on Y-chromosomal short tandem repeat (STR) polymorphisms along with reports of unusual observations and casework. Population studies were carried out on the Y-specific STR polymorphisms DYS19, DYS385 I+II, DYS389 I+II, DYS390, DYS391, DYS392, and DYS393 in population samples from North India, Turkey, and Germany. In all three populations the vast majo...

Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function eukayotic cytoskeleton. We developed pair PCR primers specifically amplified tubulysin biosynthetic genes. Using these primers, eight out eighty-one strains belonging to Cystobacteraceae family harbored putative genes were screened through analysis. The selected included two Archangium gephyra, St...

Introduction and Aim: The ApoE gene polymorphisms are considered as risk factors for developing atherosclerosis related cardiovascular disease (CVD) in humans. There exists no study pertaining to polymorphism its association these disorders among the Iraqi population. Hence this study, we aimed investigate possible relationship between single nucleotide (SNPs) with prevalence of diabetes (T2DM)...

OBJECTIVES In order to develop a multiplex RT-PCR assay using the GeXP analyser for the simultaneous detection of four different NA serotypes of H5-subtype AIVs, effective to control and reduce H5 subtype of avian influenza outbreak. DESIGN Six pairs of primers were designed using conserved and specific sequences of the AIV subtypes H5, N1, N2, N6 and N8 in GenBank. Each gene-specific primer ...

Detecting rare mutant alleles in the background of wildtype DNA sequences and quantifying a specific DNA sequence in a clinical sample are two of the most challenging problems in DNA diagnostics. Successful approaches can expect important applications. For example, prenatal diagnosis of genetic disorders can be accomplished by invasive procedures such as amniocentesis, chorionic villus sampling...

We describe a semi-empirical framework that combines thermodynamic models of primer hybridization with experimentally determined elongation biases introduced by 3'-end mismatches for improving polymerase chain reaction (PCR)-based sequence discrimination. The framework enables rational and automatic design of primers for optimal targeting of one or more sequences in ensembles of nearly identica...

This report describes a single-step extension approach suitable for high-throughput single-nucleotide polymorphism typing applications. The method relies on extension of paired allele-specific primers and we demonstrate that the reaction kinetics were slower for mismatched configurations compared with matched configurations. In our approach we employ apyrase, a nucleotide degrading enzyme, to a...